葡聚糖凝膠LH-20操作步驟產(chǎn)品應(yīng)用:
對于一些相對特殊的產(chǎn)品,我們會(huì)提供產(chǎn)品說明書,里面會(huì)對產(chǎn)品溶解性,及我們所能了解的產(chǎn)品應(yīng)用信息,實(shí)驗(yàn)方案進(jìn)行描述,您可以作為參考。對于常見試劑,因?yàn)閼?yīng)用廣泛,一般不提供說明書。如果您在實(shí)驗(yàn)過程中遇到了問題,歡迎與我們討論,我們將盡力幫您解決。
產(chǎn)品名稱:葡聚糖凝膠LH-20操作步驟
英文名字:Sephadex LH-20
應(yīng)用:利用分子篩作用,適合用有機(jī)溶劑分離嗜脂性分子,可以非常經(jīng)濟(jì)地大規(guī)模制備各種天然產(chǎn)物;如:類固醇、萜類、脂類以及小分子多肽等
性狀:白色微球,多孔
凝膠類型:凝膠過濾填料,親脂型
結(jié)構(gòu)成分:交聯(lián)葡聚糖
粒徑: 100-200目
工作PH值:2-11
操作溫度:4-40℃
排阻極限:4~5KD(與所用溶劑有關(guān))
球蛋白分離范圍:100~4000
保存條件:4-25℃
葡聚糖凝膠LH-20操作步驟分裝及溶液配制:
您收到貨物后不要自己進(jìn)行分包,因?yàn)榉职h(huán)境、包裝材料等因素可能導(dǎo)致分包后的產(chǎn)品變質(zhì);如您有特殊包裝要求,請?jiān)谟嗁彆r(shí)候與我們客服代表闡明,當(dāng)然價(jià)格會(huì)做適當(dāng)調(diào)整。對于開蓋后,長期未使用的,請務(wù)必重新密封好,建議Parafilm封口膜,并按照相應(yīng)儲(chǔ)存條件使用。如果放置時(shí)間過長,超過產(chǎn)品有效期,建議您重新購買,以免影響實(shí)驗(yàn)質(zhì)量。
葡聚糖凝膠LH-20操作步驟儲(chǔ)存條件:
大部分的試劑產(chǎn)品是比較穩(wěn)定的,只要您嚴(yán)格按照產(chǎn)品包裝或者說明書上的儲(chǔ)存條件進(jìn)行儲(chǔ)存,*可以保證其在有效期內(nèi)的正常使用。一般常見儲(chǔ)存條件有零下XX攝氏度,2-8攝氏度,常溫等。特別提醒,對于某些酶類制品,如果儲(chǔ)存條件是2-8攝氏度,不要儲(chǔ)存在零下,否則容易失活,對于某些需要嚴(yán)格避光,防潮,充氮?dú)獗Wo(hù)的,接觸空氣或者過分暴露非常容易氧化或者受潮變質(zhì)。
低溫儲(chǔ)存產(chǎn)品,運(yùn)輸途中冷藏問題:
對于需要低溫儲(chǔ)存的產(chǎn)品,我們在運(yùn)輸時(shí)候都會(huì)采用冷藏包裝,但是部分由于外界氣溫過高,運(yùn)輸時(shí)間較長,對于普通冷藏包裝,途中可能會(huì)溫度升高,這些都是沒有問題的,因?yàn)檫@種普通冷藏只適用于那些短期內(nèi)(2周)高溫依然穩(wěn)定的產(chǎn)品,建議低溫的儲(chǔ)存條件,只是長期儲(chǔ)存時(shí)候的條件,您可以放心使用。對于某些特殊的產(chǎn)品,如抗體,我們會(huì)采用干冰冷藏包裝,確保全程低溫。公司都對產(chǎn)品性狀進(jìn)行了嚴(yán)格的加速穩(wěn)定測試,行業(yè)經(jīng)驗(yàn)豐富。
葡聚糖凝膠LH-20操作步驟儲(chǔ)存條件:避光、干燥陰涼處封閉貯存,嚴(yán)禁與有毒、有害物品混放、混運(yùn)。本品為非危險(xiǎn) 產(chǎn)品可按一般化學(xué)品運(yùn)輸,輕搬動(dòng)輕放,防止日曬、雨淋!受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運(yùn)輸條件比較苛刻。
antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
Function : The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 500g 瓶 Metaphosphoric acid 偏磷酸
100g 瓶 Metaphosphoric acid 偏磷酸
25g 瓶 Calcium phytate 植酸鈣
100mg 支 Phospho(enol)pyruvic acid 磷酸烯醇式丙酮酸
胺
5g 瓶 1.4Diaminobutane (5.7ml) 腐胺
100ml 瓶 Formamide,Deionized 去離子甲酰胺
100ml 瓶 Formamide 甲酰胺
5g 瓶 Iodoacetamide 碘代乙酰胺
1g 瓶 O-Dianisidine dihydrochloride 鄰聯(lián)茴香胺
1g 支 Spermidie 亞精胺
葡聚糖凝膠LH-20操作步驟1g 支 Spermidie 3HCL 亞精胺鹽酸鹽 精脒
1g 支 Spermine 精胺
層析介質(zhì)
25ml 瓶 Arginine Sepharose 4B 精氨酸-瓊脂糖凝膠
100g 瓶 Cellulose DE-52 纖維素 DE-52