PlantScreen高通量植物表型成像分析平臺(tái)（傳送帶版）（二）

10.根系成像分析

·RhizoTron根窗技術(shù)，全自動(dòng)成像分析，標(biāo)配根窗44x29.5x5.8cm（高x寬x厚度）

·不僅可對(duì)根系成像分析，還可對(duì)地上苗（shoot）進(jìn)行成像分析，苗高*50cm

·新一代CMOS傳感器，分辨率12.3MP

·均一LED光源

·3層定位（頂部、中部、底部）根系澆灌系統(tǒng)（選配），3個(gè)水箱獨(dú)立運(yùn)行

·測(cè)量參數(shù)包括：根深（或高度）、根冠寬度、高度與寬度比值、根冠面積、根冠緊實(shí)度、根系總長(zhǎng)、軸對(duì)稱(chēng)性、根尖數(shù)、根節(jié)數(shù)等

11.自動(dòng)澆灌與稱(chēng)重單元

·測(cè)量參數(shù)：實(shí)際重量、澆水體積、*終重量、每個(gè)培養(yǎng)盆的相對(duì)重量

·操作指令：每個(gè)培養(yǎng)盆澆相同量的水（克數(shù)或者實(shí)際重量的百分比）；保持相對(duì)重量；自定義每個(gè)培養(yǎng)盆的澆灌量模擬不同干旱或者內(nèi)澇脅迫；稱(chēng)重前自動(dòng)零校準(zhǔn)，還可通過(guò)已知重量（如砝碼）物品自動(dòng)進(jìn)行再校準(zhǔn)

·每個(gè)培養(yǎng)盆的澆水量、日期、時(shí)間可分別程序控制記錄以創(chuàng)建不同干旱脅迫梯度等，并且與整個(gè)系統(tǒng)的表型大數(shù)據(jù)無(wú)縫結(jié)合分析

·稱(chēng)重精度：大型植物±2g，小型植物±0.2g

·澆灌單元：流速3L/min，澆灌口高度可自動(dòng)上下前后調(diào)整，保證澆灌位置

12.自動(dòng)化植物傳送系統(tǒng)

·傳送植物大?。焊鶕?jù)客戶(hù)需求，可達(dá)200cm

·傳送帶容納量：50盆植物（1000株小型植物），可擴(kuò)展100盆、200盆、400盆等更大容量 ；表型分析通量依不同的protocol而定，100分鐘可以完成整個(gè)系統(tǒng)載荷植物樣品的表型分析，可隨機(jī)傳送至成像室進(jìn)行成像分析、隨機(jī)澆灌

·培養(yǎng)盆：防UV聚丙烯材料，標(biāo)準(zhǔn)5L（口徑24cm）培養(yǎng)盆，可通過(guò)適配器應(yīng)用3L培養(yǎng)盆，可360度旋轉(zhuǎn)

·具備手動(dòng)載樣環(huán)（manual loading loop）以便在系統(tǒng)待機(jī)模式下手動(dòng)載樣分析實(shí)驗(yàn)、小組實(shí)驗(yàn)分析等

·具備激光植物高度測(cè)量監(jiān)測(cè)系統(tǒng)和*

·環(huán)形傳送通道：具變速箱的三相異步馬達(dá)，功率200-1000W，*負(fù)載500kg，速度150mm/s，傳送帶材料為防UV高耐用PVC

·移動(dòng)控制系統(tǒng)：*處理單元CJ2M-CPU33；數(shù)字輸入/輸出*2560點(diǎn)；輸入/輸出單元*40；溫度傳感器Pt1000，Pt100，PTC；PLC通訊百兆以太網(wǎng)；OMRON MECHATROLINK-II *16軸精確定位

·RFID標(biāo)簽和QR植物辨識(shí)系統(tǒng)，自動(dòng)讀取每個(gè)樣品托盤(pán)上的二維編碼；辨識(shí)距離2-20cm；通訊RS485；可讀取1維、2維和QR碼；配備LED光源便于弱光下辨識(shí)

·環(huán)境監(jiān)測(cè)傳感器：溫濕度傳感器、PAR光合有效輻射傳感器

·由主控制系統(tǒng)分別自動(dòng)調(diào)控每一個(gè)樣品托盤(pán)的測(cè)量時(shí)間、測(cè)量順序、測(cè)量參數(shù)、澆灌時(shí)間和澆灌量，從測(cè)量單元到培養(yǎng)室的樣品運(yùn)轉(zhuǎn)整個(gè)過(guò)程可實(shí)現(xiàn)*自動(dòng)控制，在無(wú)人值守情況下根據(jù)預(yù)設(shè)程序自行完成全部實(shí)驗(yàn)測(cè)量工作。

13.主控制表型大數(shù)據(jù)平臺(tái)

·組成：控制調(diào)度服務(wù)器、客戶(hù)端應(yīng)用服務(wù)器、數(shù)據(jù)服務(wù)器、可編程序邏輯控制器及專(zhuān)業(yè)分析軟件等，數(shù)據(jù)容量12TB

·自動(dòng)控制與分析功能：具備用戶(hù)定義、可編輯自動(dòng)測(cè)量程序（protocols），根據(jù)用戶(hù)設(shè)定程序自動(dòng)完成全部實(shí)驗(yàn)。數(shù)據(jù)結(jié)果自動(dòng)存儲(chǔ)并分析，分析的數(shù)據(jù)結(jié)果可自動(dòng)以動(dòng)態(tài)曲線的形式顯示。

·MySQL數(shù)據(jù)庫(kù)管理系統(tǒng)，可以處理?yè)碛猩锨f(wàn)條記錄的大型數(shù)據(jù)庫(kù)，支持多種存儲(chǔ)引擎，相關(guān)數(shù)據(jù)自動(dòng)存儲(chǔ)于數(shù)據(jù)庫(kù)中的不同表中

·植物編碼注冊(cè)功能：包括植物識(shí)別碼、所在托盤(pán)的識(shí)別碼等存儲(chǔ)在數(shù)據(jù)庫(kù)中，測(cè)量時(shí)自動(dòng)提取自動(dòng)讀取條形碼或RFID標(biāo)簽

·觸摸屏操作界面，在線顯示植物托盤(pán)數(shù)量、光線強(qiáng)度、分析測(cè)量狀態(tài)及結(jié)果等，輕松通過(guò)軟件*控制所有的機(jī)械部件和成像工作站

·可用默認(rèn)程序進(jìn)行所有測(cè)量，也可通過(guò)開(kāi)發(fā)工具創(chuàng)建自定義的工作過(guò)程，或者手動(dòng)操作LED光源開(kāi)啟或關(guān)閉、RGB成像、葉綠素?zé)晒獬上?、高光譜成像、紅外熱成像、3D激光掃描、稱(chēng)重及澆灌等

·葉片跟蹤監(jiān)測(cè)功能（leaf tracking）模塊，可以持續(xù)跟蹤監(jiān)測(cè)葉片的生長(zhǎng)、變化等等

·3D投射技術(shù)，可以通過(guò)高分辨率RGB鏡頭 或激光掃描構(gòu)建3D模型，通過(guò)投射技術(shù)，將與其它傳感器所得數(shù)據(jù)如葉綠素?zé)晒?、紅外熱成像溫度數(shù)據(jù)、近紅外數(shù)據(jù)、高光譜數(shù)據(jù)等投射在3D模型上一起進(jìn)行對(duì)比分析等

·允許用戶(hù)通過(guò)互聯(lián)網(wǎng)遠(yuǎn)程訪問(wèn)，進(jìn)行數(shù)據(jù)處理、下載及更改實(shí)驗(yàn)設(shè)計(jì)

·所測(cè)量的所有數(shù)據(jù)都是透明的、可以追溯的

·具備用戶(hù)權(quán)限分級(jí)功能，防止其他人員誤操作影響實(shí)驗(yàn)

·廠家遠(yuǎn)程故障診斷，軟件*升級(jí)

執(zhí)行標(biāo)準(zhǔn)：

·CE認(rèn)證標(biāo)準(zhǔn)

·CSN EN 60529 防護(hù)等級(jí)標(biāo)準(zhǔn)

·CSN 33 01 65 導(dǎo)體側(cè)識(shí)別標(biāo)準(zhǔn)

·CSN 33 2000-3 基礎(chǔ)特性標(biāo)準(zhǔn)

·CSN 33 2000-4-41ed.2 電擊保護(hù)標(biāo)準(zhǔn)

·CSN 33 2000-4-43 電源過(guò)載保護(hù)標(biāo)準(zhǔn)

·CSN 33 2000-5-51ed.2 通用規(guī)則標(biāo)準(zhǔn)

·CSN 33 2000-5-523 容許電流標(biāo)準(zhǔn)

·CSN 33 2000-5-54ed.2 接地與保護(hù)導(dǎo)體標(biāo)準(zhǔn)

·CSN EN 55011 工業(yè)、科學(xué)與醫(yī)學(xué)設(shè)備測(cè)量電磁干擾的范圍與方法

·2006/42/EG 機(jī)械指令標(biāo)準(zhǔn)

·73/23/EEG 低電壓指令標(biāo)準(zhǔn)

·2004/108/EG 電磁相容性指令標(biāo)準(zhǔn)

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附：其它表型分析平臺(tái)：

1、FKM多光譜熒光動(dòng)態(tài)顯微成像系統(tǒng)

右圖引自《Nature Plants》2016, Photonic multilayer structure of Begonia chloroplasts enhances photosynthetic efficiency by Heather M. Whitney等

2、PlantScreen-R移動(dòng)式表型分析平臺(tái)（下左圖）：用于大田植物葉綠素?zé)晒獬上穹治?、RGB成像分析、紅外熱成像分析、3D激光掃描測(cè)量分析等

3、PlantScreen臺(tái)式及移動(dòng)式植物表型分析平臺(tái)（參見(jiàn)上右圖）

1)3D RGB彩色成像分析

2)FluorCam葉綠素?zé)晒獬上穹治?/span>

3)FluorCam多光譜熒光成像分析

4)高光譜成像分析

5)紅外熱成像分析

6)PAR吸收／NDVI成像分析

7)近紅外3D成像分析

4、PlantScreen樣帶式表型分析平臺(tái)

5、PlantScreen 植物表型三維自動(dòng)掃描成像分析平臺(tái)