Product Name: SP6 RNA體外轉(zhuǎn)錄試劑盒
INTRODUCTION:
The SP6-Scribe™ Standard RNA IVT Kit is specially formulated to enable users to obtain the maximum possible yields of canonical (GAUC) RNA from an in vitro transcription (IVT) reaction. The standard 2 hour, 20 μl reaction will yield up to 90 μg of RNA from 1 μg of the control template. These yields are made possible by the high-performance properties of the SP6-Scribe enzyme. The SP6-Scribe Standard RNA IVT Kit produces exceptionally high yields of either long or short transcripts. The standard reaction can be scaled up to produce milligram amounts of RNA. In addition, an SP6-Scribe reaction can be readily modified to prepare fluorescent-, biotinylated- or digoxigeninlabeled RNA. SP6-Scribe IVT RNA can be processed into mRNA (5'-end capped and 3'-end poly(A) tailed) through the use of CELLSCRIPT's ScriptCap™ m7 G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus™ Poly(A) Polymerase Tailing Kit (available separately). CELLSCRIPT also offers the INCOGNITO™ line of transcription kits for the production of pseudouridine- & 5-methyl-cytosine-containing (GA C and GA 5mC) IVT RNA. It has been shown that -mRNAs and 5mC-mRNAs are translated into protein at higher levels and induce lower innate immune responses in human and other mammalian cells that express various RNA sensors compared to corresponding unmodified mRNAs
