Product Name: Cellscript m7G加帽試劑盒
INTRODUCTION:
The ScriptCap m7 G Capping System offers an alternative to making capped RNA by co-transcriptional capping during an in vitro transcription (IVT) reaction in which a dinucleotide cap analog (e.g.,m7 G(5')ppp(5')G) is included in place of a portion of the GTP. Provided that the 5' terminus of the RNA is not structured, the capping efficiency using the ScriptCap m7 G Capping System can approach 100%. In contrast, since the cap analog competes with GTP for initiation of transcription by the RNA polymerase, co-transcriptional capping efficiency is limited by the concentration of the cap analog and the ratio of its concentration to that of the GTP. Thus, the percentage of RNA that is capped using a cap analog in a transcription reaction is always less than 100%. The amount of capped RNA that can be made in a co-transcriptional capping reaction using a cap analog is also limited by the need to reduce GTP concentrations to permit the cap analog to compete for initiation of transcription. On the other hand, co-transcriptional capping with a cap analog can be beneficial if the RNA to be capped has a highly structured 5' terminus. Contact CELLSCRIPT to discuss the options for your project.
