主要功能

WinRHIZO 是一套用于洗根后的專(zhuān)業(yè)根系分析系統(tǒng)，可以分析根系長(zhǎng)度、直徑、面積、體積、根尖記數(shù)等，功能強(qiáng)大，操作簡(jiǎn)單，廣泛運(yùn)用于根系形態(tài)和構(gòu)造研究。

儀器內(nèi)置專(zhuān)門(mén)的雙光源照明系統(tǒng)，有效的去除了陰影和不均勻現(xiàn)象的影響，從而提供高分辨率的彩色圖像和黑白圖像，讀取TIFF，JPEG 標(biāo)準(zhǔn)格式的圖像。并采用非統(tǒng)計(jì)學(xué)方法測(cè)量計(jì)算出交叉重疊部分根系長(zhǎng)度等參量，大大提高了分析結(jié)果的準(zhǔn)確性。同時(shí)，專(zhuān)業(yè)版軟件還可以進(jìn)行更加復(fù)雜的根系顏色分析、連接分析、拓?fù)浞治龊头旨?jí)伸展分析。

測(cè)量參數(shù)

根系整體參數(shù)：總根長(zhǎng)、平均直徑、總表面積、總投影面積、總體積、根尖數(shù)、分支數(shù)和交叉數(shù)等。

根直徑等級(jí)分布參數(shù)：根長(zhǎng)、表面積、投影面積、體積、根尖數(shù)。

根系顏色分析參數(shù)：顏色類(lèi)或群的根長(zhǎng)、投影面積、表面積、平均直徑、體積、根尖計(jì)等。

根系連接分析參數(shù)：用于根系分支角度、連通性等形態(tài)研究

根系拓?fù)浞治鰠?shù)：連接數(shù)量、路徑長(zhǎng)度等研究（需要根系完整）

根系分級(jí)伸展分析參數(shù)：記錄根系整體等級(jí)分布情況（需要根系完整）

圖像中獨(dú)立單個(gè)樣品分析：?jiǎn)蝹€(gè)樣品分別的形態(tài)、連接、拓?fù)?、分?jí)伸展分析。

應(yīng)用領(lǐng)域

功能強(qiáng)大，操作簡(jiǎn)單，廣泛應(yīng)用于植物生理學(xué)、植物生態(tài)學(xué)、植物營(yíng)養(yǎng)學(xué)、農(nóng)學(xué)、園藝學(xué)、林學(xué)等領(lǐng)域的根系形態(tài)和構(gòu)造研究。

主要技術(shù)參數(shù)

主要技術(shù)參數(shù)

專(zhuān)業(yè)版

專(zhuān)業(yè)版除具備以上基本參數(shù)，還具備如下功能特征：

·   根系顏色分析：根的長(zhǎng)度、面積、體積、根尖計(jì)數(shù)、根系存活數(shù)量等研究

·   根系連接分析：用于根系分支角度、連通性等形態(tài)研究

·   根系拓?fù)浞治觯哼B接數(shù)量、路徑長(zhǎng)度等研究（需要根系完整）

·   根系分級(jí)伸展分析：記錄根系整體等級(jí)分布情況（需要根系完整）

                                                              種子分類(lèi)                                                                                                                        幼苗生長(zhǎng)定量

                                           葉片顏色分析（病理）                                      根系形態(tài)分析                                                      菌根定量分析

擬南芥版

擬南芥版除具備專(zhuān)業(yè)版的所有功能外，還具備如下功能：

·   對(duì)未接觸到一起的幼苗、根系或其他樣品，獨(dú)立進(jìn)行分區(qū)并分析；除了對(duì)圖像中的總樣品進(jìn)行統(tǒng)計(jì)分析外，對(duì)每個(gè)獨(dú)立的樣品亦可進(jìn)行統(tǒng)計(jì)分析

·   可根據(jù)圖像類(lèi)型，將樣品邊界框的長(zhǎng)度和寬度轉(zhuǎn)換成植物的高度和寬度或葉片的長(zhǎng)度和寬度

·   對(duì)圖像中每個(gè)獨(dú)立的物體自動(dòng)進(jìn)行面積計(jì)算（如果不事先對(duì)圖像進(jìn)行編輯，則重疊部分不計(jì)算，接觸到的物體算一個(gè)）

選購(gòu)指南：

系統(tǒng)由以下兩部分組成：

圖像撲捉系統(tǒng)：經(jīng)過(guò)廠家調(diào)試的標(biāo)準(zhǔn)根系掃描設(shè)備，匹配專(zhuān)門(mén)的光源，根系固定裝置等。

·   STD4800：掃描面積 22×30 cm，投影面積 20×25 cm，分辨率 4800 DPI，可分辨最小粒子 0.005 mm

·   LA2400：超大掃描面積 31x44 cm，投影面積 31x42 cm，分辨率 2400 DPI，可分辨最小粒子 0.011 mm

根系分析軟件：基本版 /標(biāo)準(zhǔn)版 /專(zhuān)業(yè)版/擬南芥版 WinRHIZO 分析軟件。

產(chǎn)地：加拿大 Regent

參考文獻(xiàn)

原始數(shù)據(jù)來(lái)源：Google Scholar

Zhang N, Shi X, Guan Z, Zhao S, Zhang F, et al. (2016) Treatment with spermidine protects chrysanthemum seedlings against salinity stress damage. Plant Physiology and Biochemistry 105: 260-270.

Zhang A, Fan D, Li Z, Xiong G, Xie Z (2016) Enhanced photosynthetic capacity by perennials in the riparian zone of the Three Gorges Reservoir Area, China. Ecological Engineering 90: 6-11.

Yu Y, Loiskandl W, Kaul H-P, Himmelbauer M, Wei W, et al. (2016) Estimation of runoff mitigation by morphologically different cover crop root systems. Journal of Hydrology 538: 667-676.

Yamamoto T, Suzuki T, Suzuki K, Adachi S, Sun J, et al. (2016) Detection of QTL for exudation rate at ripening stage in rice and its contribution to hydraulic conductance. Plant Science 242: 270-277.

Xu C, Tao H, Tian B, Gao Y, Ren J, et al. (2016) Limited-irrigation improves water use efficiency and soil reservoir capacity through regulating root and canopy growth of winter wheat. Field Crops Research 196: 268-275.

Wu J, Geilfus C-M, Pitann B, Mühling K-H (2016) Silicon-enhanced oxalate exudation contributes to alleviation of cadmium toxicity in wheat. Environmental and Experimental Botany 131: 10-18.

Win KT, Oo AZ, Ookawa T, Kanekatsu M, Hirasawa T (2016) Changes in hydraulic conductance cause the difference in growth response to short-term salt stress between salt-tolerant and -sensitive black gram (Vigna mungo) varieties. Journal of Plant Physiology 193: 71-78.

Wen Z, Shen J, Blackwell M, Li H, Zhao B, et al. (2016) Combined Applications of Nitrogen and Phosphorus Fertilizers with Manure Increase Maize Yield and Nutrient Uptake via Stimulating Root Growth in a Long-Term Experiment. Pedosphere 26: 62-73.

Wei L, Liu J, Su J, Jing G, Zhao J, et al. (2016) Effect of clipping on soil respiration components in temperate grasslａnd of Loess Plateau. European Journal of Soil Biology 75: 157-167.

Wang W-f, Zong Y-z, Zhang S-q (2016) Effects of short-term osmotic stress on leaf hydraulic conductivity and ZmPIPs mRNA accumulation in maize seedlings. Journal of Integrative Agriculture 15: 2497-2506.

Wang P, Diao F, Yin L, Huo C (2016) Absorptive roots trait plasticity explains the variation of root foraging strategies in Cunninghamia lanceolata. Environmental and Experimental Botany 129: 127-135.

Wang C-M, Xia Z-R, Wu G-Q, Yuan H-J, Wang X-R, et al. (2016) The coordinated regulation of Na+ and K+ in Hordeum brevisubulatum responding to time of salt stress. Plant Science 252: 358-366.

Vaculíková M, Vaculík M, Tandy S, Luxová M, Schulin R (2016) Alleviation of antimonate (SbV) toxicity in maize by silicon (Si). Environmental and Experimental Botany 128: 11-17.

Tripathi VK, Rajput TBS, Patel N (2016) Biometric properties and selected chemical concentration of cauliflower influenced by wastewater applied through surface and subsurface drip irrigation system. Journal of Cleaner Production 139: 396-406.

Tomè E, Ventura M, Folegot S, Zanotelli D, Montagnani L, et al. (2016) Mycorrhizal contribution to soil respiration in an apple orchard. Applied Soil Ecology 101: 165-173.

Tomar RS, Tiwari S, Vinod, Naik BK, Chａnd S, et al. (2016) Molecular and Morpho-Agronomical Characterization of Root Architecture at Seedling and Reproductive Stages for Drought Tolerance in Wheat. PLoS One 11: e0156528.

Thangthong N, Jogloy S, Pensuk V, Kesmala T, Vorasoot N (2016) Distribution patterns of peanut roots under different durations of early season drought stress. Field Crops Research 198: 40-49.

Terzaghi M, Di Iorio A, Montagnoli A, Baesso B, Scippa GS, et al. (2016) Forest canopy reduction stimulates xylem production and lowers carbon concentration in fine roots of European beech. Forest Ecology and Management 379: 81-90.

Tang B, Yin C, Wang Y, Sun Y, Liu Q (2016) Positive effects of night warming on physiology of coniferous trees in late growing season: Leaf and root. Acta Oecologica 73: 21-30.

Sun Y, Mu C, Chen Y, Kong X, Xu Y, et al. (2016) Comparative transcript profiling of maize inbreds in response to long-term phosphorus deficiency stress. Plant Physiology and Biochemistry 109: 467-481.

Sonnemann I, Buchholz Y, Rautenberg D, Wurst S (2016) Lａnd use intensity modulates the impact of root herbivores on plant interactions with above- and below-ground organisms. Agriculture, Ecosystems & Environment 219: 26-31.

Shtein I, Noy-Porat T, Eshel A (2016) Life cycle and reproductive botany of Scilla hyacinthoides, a Mediterranean geophyte. Scientia Horticulturae 201: 167-174.

Shi Y, Zhou G, Jiang Y, Wang H, Xu Z (2016) Does precipitation mediate the effects of elevated CO2 on plant growth in the grass species Stipa grandis? Environmental and Experimental Botany 131: 146-154.

Shan G, Sun Y, Cheng Q, Wang Z, Zhou H, et al. (2016) Monitoring tomato root zone water content variation and partitioning evapotranspiration with a novel horizontally-oriented dielectric sensor. Agricultural and Forest Meteorology 228–229: 85-94.

Saha S, Chakraborty D, Sehgal VK, Nain L, Pal M (2016) Long-term atmospheric CO2 enrichment impact on soil biophysical properties and root nodule biophysics in chickpea (Cicer arietinum L.). European Journal of Agronomy 75: 1-11.

Ryan MH, Kidd DR, Sandral GA, Yang Z, Lambers H, et al. (2016) High variation in the percentage of root length colonised by arbuscular mycorrhizal fungi among 139 lines representing the species subterranean clover (Trifolium subterraneum). Applied Soil Ecology 98: 221-232.

Ruiz-Yanetti S, Chirino E, Bellot J (2016) Daily whole-seedling transpiration determined by minilysimeters, allows the estimation of the water requirements of seedlings used for drylａnd afforestation. Journal of Arid Environments 124: 341-351.

Reyes-Cabrera J, Zotarelli L, Dukes MD, Rowlａnd DL, Sargent SA (2016) Soil moisture distribution under drip irrigation and seepage for potato production. Agricultural Water Management 169: 183-192.

Rees F, Sterckeman T, Morel JL (2016) Root development of non-accumulating and hyperaccumulating plants in metal-contaminated soils amended with biochar. Chemosphere 142: 48-55.

Rankl S, Gunsé B, Sieper T, Schmid C, Poschenrieder C, et al. (2016) Microbial homoserine lactones (AHLs) are effectors of root morphological changes in barley. Plant Science 253: 130-140.

Ramamoorthy P, Lakshmanan K, Upadhyaya HD, Vadez V, Varshney RK (2016) Shoot traits and their relevance in terminal drought tolerance of chickpea (Cicer arietinum L.). Field Crops Research 197: 10-27.

Qiao Y, Fan J, Wang Q (2016) Effects of farmlａnd shelterbelts on accumulation of soil nitrate in agro-ecosystems of an oasis in the Heihe River Basin, China. Agriculture, Ecosystems & Environment 235: 182-192.

Qiang-Sheng W, Ming-Qin C, Ying-Ning Z, Chu W, Xin-Hua H (2016) Mycorrhizal colonization represents functional equilibrium on root morphology and carbon distribution of trifoliate orange grown in a split-root system. Scientia Horticulturae 199: 95-102.