上海博研生物工程研究中心竭誠為您提供的產(chǎn)品和服務(wù)，期待您的來電,Elisa試劑盒，Elisa試劑盒說明書，Elisa試劑盒價格
產(chǎn)品名稱：豬乙酰*（ACh）ELISA試劑盒優(yōu)貨銷售
英文名稱：PoFAine acetylcholine,ACH ElisaKit.
產(chǎn)品編號：BYS12616B
產(chǎn)品性狀：液體
產(chǎn)品用途：科研實驗
產(chǎn)品規(guī)格：96T/48T
ELISA實驗原理: 用純化的抗體包被微孔板，制成固相載體，往包被抗體的微孔中依次加入標(biāo)本或者標(biāo)準(zhǔn)品、*化的抗體、HRP-標(biāo)記的親和素，經(jīng)過*洗滌后用底物顯色。底物在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色， 并在酸的作用下轉(zhuǎn)化成zui終的黃色。 顏色的深淺和樣品中的目標(biāo)蛋白呈正相關(guān)。用酶標(biāo)儀在（450nm）波長下測定測定O.D.值（吸光度），計算樣品濃度。.
豬乙酰*（ACh）ELISA試劑盒優(yōu)貨銷售 相關(guān)產(chǎn)品：
豚鼠免疫球蛋白E（IgE）試劑盒說明書，96T/48T*
豚鼠免疫球蛋白A（IgA）試劑盒說明書，96T/48T*
豚鼠基質(zhì)金屬蛋白酶抑制因子1（TIMP-1）試劑盒說明書，96T/48T*
豚鼠基質(zhì)金屬蛋白酶9（MMP-9）試劑盒說明書，96T/48T*
豚鼠肝細(xì)胞生長因子（HGF）試劑盒說明書，96T/48T*
豚鼠γ干擾素（IFN-γ）試劑盒說明書，96T/48T*
豚鼠端粒酶（TE）試劑盒說明書，96T/48T*
豚鼠促卵泡素（FSH）試劑盒說明書，96T/48T*
豚鼠腸脂肪酸結(jié)合蛋白（iFABP）試劑盒說明書，96T/48T*
豚鼠表皮生長因子（EGF）試劑盒說明書，96T/48T*
人PL7抗體/抗蘇氨酰tRNA合成酶（PL7）試劑盒說明書，96T/48T*
人PL12抗體/抗丙氨酰tRNA合成酶（PL12/AlaRS）試劑盒說明書，96T/48T*
人抗Mi2抗體（anti-Mi2-Ab）試劑盒說明書，96T/48T*
人抗Ku抗體（anti-Ku-Ab）試劑盒說明書，96T/48T*
人抗神經(jīng)元核抗體1型/抗Hu抗體（ANNA-1/Hu）試劑盒說明書，96T/48T*
人抗DNA酶B抗體（anti-DNase B）試劑盒說明書，96T/48T*
人抗BP180抗體（BP180-Ab）試劑盒說明書，96T/48T*
人抗BB抗體（BB-Ab）試劑盒說明書，96T/48T*
人幽門螺旋菌IgG（Hp-IgG）試劑盒說明書，96T/48T*
人可溶性血管內(nèi)皮生長因子受體2（VEGFR-2/sFLK-1）試劑盒說明書，96T/48T*
The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase（HRP） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve
