上海博研生物工程研究中心竭誠(chéng)為您提供的產(chǎn)品和服務(wù),期待您的來(lái)電,Elisa試劑盒,Elisa試劑盒說(shuō)明書,Elisa試劑盒價(jià)格
產(chǎn)品名稱:豬血栓調(diào)節(jié)蛋白(TM)ELISA試劑盒如您所愿
英文名稱:PoFAine thrombomodulin,TM ElisaKit.
產(chǎn)品編號(hào):BYS12627B
產(chǎn)品性狀:液體
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品規(guī)格:96T/48T
ELISA實(shí)驗(yàn)原理: 用純化的抗體包被微孔板,制成固相載體,往包被抗體的微孔中依次加入標(biāo)本或者標(biāo)準(zhǔn)品、*化的抗體、HRP-標(biāo)記的親和素,經(jīng)過(guò)*洗滌后用底物顯色。底物在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色, 并在酸的作用下轉(zhuǎn)化成zui終的黃色。 顏色的深淺和樣品中的目標(biāo)蛋白呈正相關(guān)。用酶標(biāo)儀在(450nm)波長(zhǎng)下測(cè)定測(cè)定O.D.值(吸光度),計(jì)算樣品濃度。.
豬血栓調(diào)節(jié)蛋白(TM)ELISA試劑盒如您所愿 相關(guān)產(chǎn)品:
兔子巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)試劑盒說(shuō)明書,96T/48T*
兔子神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)試劑盒說(shuō)明書,96T/48T*
豚鼠白介素4(IL-4)試劑盒說(shuō)明書,96T/48T*
豚鼠免疫球蛋白G(IgG)試劑盒說(shuō)明書,96T/48T*
豚鼠血清總補(bǔ)體(CH50)試劑盒說(shuō)明書,96T/48T*
豚鼠組胺(HIS)試劑盒說(shuō)明書,96T/48T*
豚鼠轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)試劑盒說(shuō)明書,96T/48T*
豚鼠腫瘤壞死因子α(TNF-α)試劑盒說(shuō)明書,96T/48T*
豚鼠生長(zhǎng)激素(GH)試劑盒說(shuō)明書,96T/48T*
豚鼠內(nèi)皮素1(ET-1)試劑盒說(shuō)明書,96T/48T*
*重鏈(IgH)試劑盒說(shuō)明書,96T/48T*
*重鏈可變區(qū)(IgHV)試劑盒說(shuō)明書,96T/48T*
人多免疫球蛋白受體(poly-IgR)試劑盒說(shuō)明書,96T/48T*
*輕鏈kappa抗體(κ-IgLC)試劑盒說(shuō)明書,96T/48T*
人表面膜免疫球蛋白A(mIgA)試劑盒說(shuō)明書,96T/48T*
人果糖1,6二磷酸醛縮酶(FDA)試劑盒說(shuō)明書,96T/48T*
*j鏈(Ig-j)試劑盒說(shuō)明書,96T/48T*
人抗核周因子抗體(APF)試劑盒說(shuō)明書,96T/48T*
人*原激活肽(TAP)試劑盒說(shuō)明書,96T/48T*
人補(bǔ)體因子H(CFH)試劑盒說(shuō)明書,96T/48T*
The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve