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更新時(shí)間:2023-06-21 08:20:57瀏覽次數(shù):75次
聯(lián)系我時(shí),請(qǐng)告知來自 儀表網(wǎng)A549細(xì)胞株人非小細(xì)胞肺癌細(xì)胞系是1972年由GiardDJ通過肺癌組織移植培養(yǎng)建系的,源自一位58歲的白人男性。A549能通過胞苷二磷脂酰途徑合成富含不飽和脂肪酸的;角蛋白陽性。
A549細(xì)胞株
A549細(xì)胞株 人非小細(xì)胞肺癌細(xì)胞系是1972年由GiardDJ通過肺癌組織移植培養(yǎng)建系的,源自一位58歲的白人男性。A549能通過胞苷二磷脂酰途徑合成富含不飽和脂肪酸的;角蛋白陽性。
細(xì)胞名稱:人非小細(xì)胞肺癌細(xì)胞
細(xì)胞別稱: A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549-ATCC; hA549
細(xì)胞簡(jiǎn)稱:A549細(xì)胞株
產(chǎn)品貨號(hào):TCH-C116
種屬來源:人
組織來源:肺
特征:肺癌
細(xì)胞形態(tài):上皮細(xì)胞樣
生長(zhǎng)特性:貼壁生長(zhǎng)
培養(yǎng)體系:D/F12+10%FBS+1%P/S
A549細(xì)胞培養(yǎng)基貨號(hào):TCH-G116
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測(cè):細(xì)菌、真菌、支原體檢測(cè)均為陰性
實(shí)驗(yàn)室儀器及培養(yǎng)環(huán)境:CO2培養(yǎng)箱 細(xì)胞培養(yǎng)瓶 細(xì)胞培養(yǎng)皿
參考文獻(xiàn):"Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed:
Giard DJ, et al. In vitro c*tion of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365
Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383
St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830
Horikami SM, et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522
Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475"
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