脂氧合酶同源結(jié)構(gòu)域1抗體LOXHD1簡(jiǎn)單介紹：

產(chǎn)品編號(hào)：BYK-18343R

英文名稱：Anti-LOXHD1
中文名稱：脂氧合酶同源結(jié)構(gòu)域1抗體

別名：DFNB77; FLJ32670; LH2D1; Lipoxygenase homology domain-containing protein 1; Lipoxygenase homology domains 1; LOXH1_HUMAN; LOXHD1.

背景介紹：This gene encodes a highly conserved protein consisting entirely of PLAT (polycystin/lipoxygenase/alpha-toxin) domains, thought to be involved in targeting proteins to the plasma membrane. Studies in mice show that this gene is expressed in the mechanosensory hair cells in the inner ear, and mutations in this gene lead to auditory defects, indicating that this gene is essential for normal hair cell function. Screening of human families segregating deafness identified a mutation in this gene which causes DFNB77, a progressive form of autosomal-recessive nonsyndromic hearing loss (ARNSHL). Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. 

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

脂氧合酶同源結(jié)構(gòu)域1抗體LOXHD1產(chǎn)品信息：

染色失敗的幾種原因

1.所染的全部切片均為陰性結(jié)果，包括陽(yáng)性對(duì)照在內(nèi)，全部呈陰性反應(yīng)，原因可能是：（1）染色未按嚴(yán)格操作步驟進(jìn)行；（2）漏加一種抗體，或抗體失效；（3）緩沖液內(nèi)含*，抑制了酶的活性；（4）底物中所加H2O2量少或失效；復(fù)染或脫水劑使用不當(dāng)。

2.所有切片均呈弱陽(yáng)性反應(yīng)：（1）切片在染色過程中抗體過濃，或干燥了；（2）緩沖液配制中未加lu化鈉或pH不準(zhǔn)確，洗滌不*；（3）使用已變色的呈色底物溶液，或呈色反應(yīng)時(shí)間過長(zhǎng)；（4）抗體溫育的時(shí)間過長(zhǎng)；（5）H2O2濃度過高，呈色速度過快；（6）粘附劑太厚。

3.所有切片背景過深：（1）未用酶消化處理切片；（2）切片或涂片過厚；（3）漂洗不夠；（4）底物呈色反應(yīng)過久；（5）蛋白質(zhì)封閉不夠或所用血清溶血；（6）使用全血清抗體稀釋不夠。

4.陽(yáng)性對(duì)照染色良好，檢測(cè)的陽(yáng)性標(biāo)本呈陰性反應(yīng)，固定和處理不當(dāng)是zui常見的原因。對(duì)于陽(yáng)性結(jié)果的定量判斷，常規(guī)方法是根據(jù)呈色深淺和陽(yáng)性細(xì)胞數(shù)量分類計(jì)數(shù)，以（-）、+、++、+++等分級(jí)和計(jì)數(shù)統(tǒng)計(jì)。

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