滬鼎產(chǎn)品文獻(xiàn):人IL-6,TNF-α,iNOS ELISA試劑盒引用文獻(xiàn)

【文獻(xiàn)標(biāo)題】Rapeseed protein-derived ACE inhibitory peptides LY, RALP and GHS show

antioxidant and anti-inflammatory effects on spontaneously hypertensive rats

【作者】Rong He，Yujiao Wang，Yijie Yang，et.al

【作者單位】南京財經(jīng)大學(xué)（Nanjing University of Finance and Economics）

【文獻(xiàn)中引用產(chǎn)品】

人白細(xì)胞介素6(IL-6)ELISA試劑盒

人腫瘤壞死因子α(TNF-α)ELISA試劑盒

人誘導(dǎo)型一氧化氮合成酶(iNOS)ELISA試劑盒

【關(guān)鍵詞】Rapeseed peptide，ACE inhibition，Anti-inflammatory effect，Cytokine，Oxidative  stress

【DOI】doi.org/10.1016/j.jff.2019.02.031

【影響因子(IF)】5.9

【出版期刊】《Journal of Functional Foods》

【產(chǎn)品原文引用】

Materials and reagents

RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, and hematoxylin and eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit and Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit and Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) and Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).

滬鼎產(chǎn)品文獻(xiàn):人Tau蛋白(TAU)ELISA試劑盒引用文獻(xiàn)

【文獻(xiàn)標(biāo)題】Effect of erythropoietin combined with hypothermia on serum tau protein levels and neurodevelopmental outcome in neonates with hypoxic-ischemic encephalopathy

【作者】Hong-yan Lv，Su-jing Wu，Qiu-li Wang，et.al

【作者單位】邯鄲市邯鄲婦幼保健院（Handan Maternal and Child Health Care Hospital, Handan）

【文獻(xiàn)中引用產(chǎn)品】

人Tau蛋白(TAU)ELISA試劑盒

【關(guān)鍵詞】nerve regeneration; erythropoietin; hypothermia; hypoxic-ischemic encephalopathy; neonate; tau protein; biomarkers; prognosis; neuroprotection; neural regeneration

【DOI】doi.org/10.4103/1673-5374.217338

【影響因子(IF)】3.6

【出版期刊】《NEURAL REGENERATION RESEARCH》

【產(chǎn)品原文引用】

Serum tau protein measurement

Blood samples were taken from the radial vein at 24 hours after birth (before EPO treatment) and 4, 8, and 12 days after EPO treatment. Blood samples were placed in a test tube (specifications: 5 mL, No A06277602; Hebi City Zhong Xing Medical Supplies, Hebi, China), maintained without stirring for 30 minutes at room temperature, and then centrifuged in a L-400 low-speed automatic balancing centrifuge (Hunan Xiangyi Laboratory Instrument Development, Changsha, Hunan Province, China) at 3,000 r/min for 15 minutes.Serum was removed into another test tube and placed in a freezer at ?70°C until use. To avoid any influence on the results, blood transfusions to the neonates were avoided before collecting blood samples. Serum tau protein was detected using an enzyme-linked immunosorbent assay. The kit was provided by the Shanghai HuDing Biological Science and Technology Co., Ltd.

滬鼎產(chǎn)品文獻(xiàn):人白介素4,6,8,10,HGF,TIMP-1等ELISA試劑盒引用文獻(xiàn)

【文獻(xiàn)標(biāo)題】水凝膠三維培養(yǎng)對人羊膜間充質(zhì)干細(xì)胞特性及旁分泌效應(yīng)的影響

【作者】王 旗，楊曉雙，王達(dá)利

【作者單位】遵義醫(yī)科大學(xué)

【文獻(xiàn)中引用產(chǎn)品】

人白細(xì)胞介素4(IL-4)ELISA試劑盒

人白細(xì)胞介素6(IL-6)ELISA試劑盒

人白細(xì)胞介素8(IL-8)ELISA試劑盒

人白細(xì)胞介素10(IL-10)ELISA試劑盒

人肝細(xì)胞生長因子(HGF)ELISA試劑盒

人金屬蛋白酶組織抑制因子-1(TIMP-1)ELISA試劑盒

人基質(zhì)金屬蛋白酶1(MMP-1)ELISA檢測試劑盒

人表皮生長因子（EGF）ELISA試劑盒

人轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒

人堿性成纖維細(xì)胞生長因子(bFGF)ELISA試劑盒

人角化細(xì)胞生長因子(KGF)ELISA試劑盒

人血管內(nèi)皮生長因子(VEGF)ELISA試劑盒

【關(guān)鍵詞】水凝膠；羊膜間充質(zhì)干細(xì)胞；二維培養(yǎng)；三維培養(yǎng)；創(chuàng)面修復(fù)；旁分泌效應(yīng)

【DOI】10.3969/j.issn.2095-4344.2299 

【影響因子】1.1

【出版期刊】《中國組織工程研究》

【產(chǎn)品原文引用】

ELISA法檢測 :

ELISA法檢測兩種培養(yǎng)條件下hAMSCs旁分泌創(chuàng)面愈合相關(guān)因子的蛋白表達(dá)情況。將hAMSCs按照2×104

/cm2接種于兩種不同培養(yǎng)條件的24孔板上。根據(jù)二維培養(yǎng)的細(xì)胞情況，細(xì)胞貼壁后用PBS清洗兩種培養(yǎng)條件下的hAMSCs并更換新鮮培養(yǎng)基，放入細(xì)胞培養(yǎng)箱繼續(xù)培養(yǎng)48 h。48 h后收集兩種細(xì)胞培養(yǎng)液， 1 000×g離心20 min，取上清。根據(jù)Elisa試劑盒(上海滬鼎)說明書進(jìn)行加樣，在450 nm波長處測定各孔的吸光度值。繪制標(biāo)準(zhǔn)曲線，計算白細(xì)胞介素4、白細(xì)胞介素6、白細(xì)胞介素8、白細(xì)胞介素10、肝細(xì)胞生長因子、金屬蛋白酶組織抑制因子、基質(zhì)金屬蛋白酶、表皮生長因子、轉(zhuǎn)化生長因子β1、堿性成纖維細(xì)胞因子、角化細(xì)胞生長因子、血管內(nèi)皮生長因子的相應(yīng)濃度。

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