小鼠*/*蛋白磷酸酶(STK)ELISa免疫檢測(cè)試劑盒操作步驟保存及運(yùn)輸溫度：2-8℃低溫避光保存公司批次ELISA試劑盒供應(yīng)，常見ELISA試劑盒享受折扣優(yōu)惠。ELISA試劑盒棋盤試驗(yàn)的操作方法：（1）將抗原按一定的梯度倍比稀釋后，按照ELISA從上到下（或者從左到右）的順序包被。（2）將抗體按一定的梯度倍比稀釋后按照ELISA從左到右或者從上到下加入。（3）二抗的稀釋倍數(shù)是一定的，然后顯色，讀值。
產(chǎn)品名稱：小鼠*/*蛋白磷酸酶(STK)ELISa免疫檢測(cè)試劑盒操作步驟
英文名稱：Mouseserine/threonineproteinkinase,STKELISA Kit
性狀：盒裝液體。 
保存溫度： 2~8℃。 
檢測(cè)范圍：見說明書靈敏度（以隨貨英文說明書為主）。 
說明書:說明書隨貨發(fā)送，您也可以直接我司在線銷售人員索取。
樣品收集、處理及保存方法
1、  血清-----操作過程中避免任何細(xì)胞刺激。使用不含熱原和內(nèi)毒素的試管。收集血液后，1000×g離心10分鐘將血清和紅細(xì)胞迅速小心地分離。
2、  血漿-----EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè)。1000×g離心30分鐘去除顆粒。
3、  細(xì)胞上清液---1000×g離心10分鐘去除顆粒和聚合物。
4、  保存------如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
小鼠*/*蛋白磷酸酶(STK)ELISa免疫檢測(cè)試劑盒操作步驟注意事項(xiàng)
1．  試劑應(yīng)按標(biāo)簽說明書儲(chǔ)存，使用前恢復(fù)到室溫。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。
2．  實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。
3．  不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用。保質(zhì)前使用。
4．  使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。
5．  使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6．  洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。
7．  底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。
8．  加入試劑的順序應(yīng)*，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。
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ELISA Kit for Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B)規(guī)格：48T/96T
ELISA Kit for Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E)規(guī)格：48T/96T
ELISA Kit for Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H)規(guī)格：48T/96T
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ELISA Kit for ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)規(guī)格：48T/96T
ELISA Kit for ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)規(guī)格：48T/96T
ELISA Kit for Solute Carrier Family 30, Member 3 (SLC30A3)規(guī)格：48T/96T
ELISA Kit for Solute Carrier Family 30, Member 5 (SLC30A5)規(guī)格：48T/96T
ELISA Kit for Solute Carrier Family 30, Member 6 (SLC30A6)規(guī)格：48T/96T
ELISA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b)規(guī)格：48T/96T
ELISA Kit for Triosephosphate Isomerase 1 (TPI1)規(guī)格：48T/96T
ELISA Kit for Sepiapterin Reductase (SPR)規(guī)格：48T/96T
ELISA Kit for Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)規(guī)格：48T/96T
ELISA Kit for Protection Of omeres 1 Homolog (POT1)規(guī)格：48T/96T
ELISA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)規(guī)格：48T/96T
ELISA Kit for FK506 Binding Protein Like Protein (FKBPL)規(guī)格：48T/96T
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 4 (WNT4)規(guī)格：48T/96T
ELISA Kit for RCC1 And BTB Domain Containing Protein 2 (RCBTB2)規(guī)格：48T/96T
ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2)規(guī)格：48T/96T
ELISA Kit for SET Domain Containing Protein 7 (SETD7)規(guī)格：48T/96T
ELISA Kit for Hermansky Pudlak Syndrome Protein 6 (HPS6)規(guī)格：48T/96T
ELISA Kit for Protease, Serine 50 (PRSS50)規(guī)格：48T/96T
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ELISA Kit for Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B)規(guī)格：48T/96T
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 9B (WNT9B)規(guī)格：48T/96T
ELISA Kit for T-Box Protein 21 (TBX21)規(guī)格：48T/96T
ELISA Kit for Mucin 12, Cell Surface Associated (MUC12)規(guī)格：48T/96T
ELISA Kit for ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4)規(guī)格：48T/96T
ELISA Kit for Echinoderm Microtubule Associated Protein Like Protein 2 (EML2)規(guī)格：48T/96T
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ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3)規(guī)格：48T/96T
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ELISA Kit for Superoxide Dismutase, Copper Chaperone (SOD4)規(guī)格：48T/96T
ELISA Kit for Caspase 3 (P3)規(guī)格：48T/96T
ELISA Kit for Complement Receptor 2 (CR2)規(guī)格：48T/96T
ELISA Kit for Programmed Cell Death Protein 1 (PDCD1)規(guī)格：48T/96T
ELISA Kit for Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)規(guī)格：48T/96T
ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)規(guī)格：48T/96T
ELISA Kit for Parathyroid Hormone Related Protein (PTHrP)規(guī)格：48T/96T
ELISA Kit for Integrin Alpha 2 (ITGa2)規(guī)格：48T/96T