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小鼠主要組織相容性復(fù)合體Ⅱ類(MHC-Ⅱ/H-2ⅡELISa免疫檢測試劑盒操作步驟檢測范圍:
人、綿羊、小鼠、大鼠、豬、兔、山羊、牛、馬、豬、其它動物細(xì)胞因子、植物細(xì)胞因子、骨代謝、細(xì)胞凋亡、激素內(nèi)分泌、活性多肽、肝纖維化、自身抗體、血栓與止血、腫瘤、自身抗體科研Elisa檢測試劑盒。
產(chǎn)品名稱:小鼠主要組織相容性復(fù)合體Ⅱ類(MHC-Ⅱ/H-2ⅡELISa免疫檢測試劑盒操作步驟
英文名稱:Mousemajorhistocompatibilitycomplex-Ⅱ,MHC-Ⅱ/H-2ⅡELISA Kit
產(chǎn)品性狀:盒裝液體。
運輸條件:2-8℃低溫運輸,用干冰或者冰袋低溫運輸。
待檢樣本:體液、血清、血漿、細(xì)胞培養(yǎng)上清液、尿液、組織勻漿、心房水標(biāo)本等等。
小鼠主要組織相容性復(fù)合體Ⅱ類(MHC-Ⅱ/H-2ⅡELISa免疫檢測試劑盒操作步驟原理:ELISA是以免疫學(xué)反應(yīng)為基礎(chǔ),將抗原、抗體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗技術(shù)。
ELISA試劑盒檢測類型:
雙抗體夾心法測抗原、雙抗原夾心法測抗體、間接法測抗體、競爭法測抗體、競爭法測抗原、捕獲包被法測抗體、ABS-ELISA法。
類型和操作步驟
ELISA可用于測定抗原,也可用于測定抗體。在這種測定方法中有3種必要的試劑:
①固相的抗原或抗體,
②酶標(biāo)記的抗原或抗體,
③酶作用的底物。
小鼠主要組織相容性復(fù)合體Ⅱ類(MHC-Ⅱ/H-2ⅡELISa免疫檢測試劑盒操作步驟根據(jù)試劑的來源和標(biāo)本的性狀以及檢測的具備條件,可設(shè)計出各種不同類型的檢測方法。
(一)雙抗體夾心法、
(二)一步法、
(三)間接法測抗體、
(四)競爭法。
優(yōu)點:
1、*抗體——高效、靈敏、特異
2、規(guī)范包被操作——吸附均勻,吸附性好,空白值低,孔底透明度高
3、*的優(yōu)化方案——重復(fù)性高,可靠性強(qiáng)
4、技術(shù)服務(wù)要求:專業(yè),規(guī)范,高效
5、適用于體液、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等多種類型的樣本適用范圍:此試劑盒僅用于科研實驗,禁用于臨床
技術(shù)原理:
(1). 抗原或抗體的固相化及抗原或抗體的酶標(biāo)記。
(2). 結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性。
(3). 酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性。
(4). 受檢標(biāo)本與固相載體表面的抗原或抗體起反應(yīng)。再加入酶標(biāo)記的抗原或抗體,也通過反應(yīng)而結(jié)合在固相載體上。
(5). 此時固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例。
(6). 加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析。測定方法具有很高的敏感度(pg-ng/ml水平),并且重復(fù)性好。以免疫學(xué)反應(yīng)為基礎(chǔ),將抗原、抗體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗技術(shù)。
ELISA Kit for Cytochrome P450 11B1 (CYP11B1)規(guī)格:48T/96T
ELISA Kit for Deiodinase, Iodothyronine, Type I (DIO1)規(guī)格:48T/96T
ELISA Kit for Deiodinase, Iodothyronine, Type III (DIO3)規(guī)格:48T/96T
ELISA Kit for RNA Binding Motif Protein 20 (RBM20)規(guī)格:48T/96T
ELISA Kit for RNA Binding Motif Protein 24 (RBM24)規(guī)格:48T/96T
ELISA Kit for Poliovirus Receptor (PVR)規(guī)格:48T/96T
ELISA Kit for RNA Binding Motif Protein 38 (RBM38)規(guī)格:48T/96T
ELISA Kit for Matrix Metalloproteinase 1 (MMP1)規(guī)格:48T/96T
ELISA Kit for Thyroid Hormone Receptor Alpha (THRa)規(guī)格:48T/96T
ELISA Kit for Thyroid Hormone Receptor Alpha (THRa)規(guī)格:48T/96T
ELISA Kit for Deiodinase, Iodothyronine, Type II (DIO2)規(guī)格:48T/96T
ELISA Kit for Desmocollin 2 (DSC2)規(guī)格:48T/96T
ELISA Kit for Gap Junction Protein Beta 1 (GJb1)規(guī)格:48T/96T
ELISA Kit for V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2)規(guī)格:48T/96T
ELISA Kit for SPARC Like Protein 1 (SPARCL1)規(guī)格:48T/96T
ELISA Kit for Misato Homolog 1 (MSTO1)規(guī)格:48T/96T
ELISA Kit for Proteinase 3 (PR3)規(guī)格:48T/96T
ELISA Kit for Neurofilament, Light Polypeptide (NEFL)規(guī)格:48T/96T
ELISA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC)規(guī)格:48T/96T
ELISA Kit for Acyloxyacyl Hydrolase (AOAH)規(guī)格:48T/96T
ELISA Kit for Astacin Like Metallo Endopeptidase (ASTL)規(guī)格:48T/96T
ELISA Kit for Casein Beta (CSN2)規(guī)格:48T/96T
ELISA Kit for Cysteine Rich Secretory Protein 3 (CRISP3)規(guī)格:48T/96T
ELISA Kit for 2',5'-Oligoadenylate Synthetase 2 (OAS2)規(guī)格:48T/96T
ELISA Kit for 2',5'-Oligoadenylate Synthetase 3 (OAS3)規(guī)格:48T/96T
ELISA Kit for Procalcitonin (PCT)規(guī)格:48T/96T
ELISA Kit for Interleukin 10 Receptor Alpha (IL10Ra)規(guī)格:48T/96T
ELISA Kit for Death Associated Protein Kinase 1 (DAPK1)規(guī)格:48T/96T
ELISA Kit for Fibroblast Activation Protein Alpha (FAPa)規(guī)格:48T/96T
ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1)規(guī)格:48T/96T
小鼠主要組織相容性復(fù)合體Ⅱ類(MHC-Ⅱ/H-2ⅡELISa免疫檢測試劑盒操作步驟ELISA Kit for Escherichia coli Protein (ECP)規(guī)格:48T/96T
ELISA Kit for Fibrinogen Like Protein 1 (FGL1)規(guī)格:48T/96T
ELISA Kit for Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1)規(guī)格:48T/96T
ELISA Kit for Death Associated Protein Kinase 3 (DAPK3)規(guī)格:48T/96T
ELISA Kit for Nuclear Mitotic Apparatus Protein 1 (NUMA1)規(guī)格:48T/96T
ELISA Kit for S100 Calcium Binding Protein A2 (S100A2)規(guī)格:48T/96T
ELISA Kit for Angiotensin 1-7 (Ang1-7)規(guī)格:48T/96T
ELISA Kit for Kinesin Family, Member 14 (KIF14)規(guī)格:48T/96T
ELISA Kit for V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)規(guī)格:48T/96T
ELISA Kit for Secretogranin III (SCG3)規(guī)格:48T/96T
ELISA Kit for Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1)規(guī)格:48T/96T
ELISA Kit for Ornithine Decarboxylase (ODC)規(guī)格:48T/96T
ELISA Kit for Transglutaminase 3, Epidermal (TGM3)規(guī)格:48T/96T
ELISA Kit for Transglutaminase 1, Keratinocyte (TGM1)規(guī)格:48T/96T
ELISA Kit for Interferon Alpha (IFNa)規(guī)格:48T/96T
ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)規(guī)格:48T/96T
ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)規(guī)格:48T/96T
ELISA Kit for Interleukin 31 (IL31)規(guī)格:48T/96T
ELISA Kit for Pleiotrophin (PTN)規(guī)格:48T/96T
ELISA Kit for Pleiotrophin (PTN)規(guī)格:48T/96T
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