小鼠E選擇素(E-Selectin/CD62E)ELISa免疫檢測試劑盒保存保存及運輸溫度：2-8℃低溫避光保存公司批次ELISA試劑盒供應(yīng)，常見ELISA試劑盒享受折扣優(yōu)惠。ELISA試劑盒棋盤試驗的操作方法：（1）將抗原按一定的梯度倍比稀釋后，按照ELISA從上到下（或者從左到右）的順序包被。（2）將抗體按一定的梯度倍比稀釋后按照ELISA從左到右或者從上到下加入。（3）二抗的稀釋倍數(shù)是一定的，然后顯色，讀值。
產(chǎn)品名稱：小鼠E選擇素(E-Selectin/CD62E)ELISa免疫檢測試劑盒保存
英文名稱：MouseE-SelectinELISA Kit
性狀：盒裝液體。 
保存溫度： 2~8℃。 
檢測范圍：見說明書靈敏度（以隨貨英文說明書為主）。 
說明書:說明書隨貨發(fā)送，您也可以直接我司在線銷售人員索取。
樣品收集、處理及保存方法
1、  血清-----操作過程中避免任何細(xì)胞刺激。使用不含熱原和內(nèi)毒素的試管。收集血液后，1000×g離心10分鐘將血清和紅細(xì)胞迅速小心地分離。
2、  血漿-----EDTA、檸檬酸鹽、肝素血漿可用于檢測。1000×g離心30分鐘去除顆粒。
3、  細(xì)胞上清液---1000×g離心10分鐘去除顆粒和聚合物。
4、  保存------如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
注意事項
1．  試劑應(yīng)按標(biāo)簽說明書儲存，使用前恢復(fù)到室溫。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。
2．  實驗中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。
3．  不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用。保質(zhì)前使用。
4．  使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時，避免使用帶金屬部分的加樣器。
5．  使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6．  洗滌酶標(biāo)板時應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。
7．  底物A應(yīng)揮發(fā)，避免長時間打開蓋子。底物B對光敏感，避免長時間暴露于光下。避免用手接觸，有毒。實驗完成后應(yīng)立即讀取OD值。
8．  加入試劑的順序應(yīng)*，以保證所有反應(yīng)板孔溫育的時間一樣。
 

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