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小鼠載脂蛋白H(Apo-H)ELISa免疫檢測試劑盒操作步驟保存及運(yùn)輸溫度:2-8℃低溫避光保存公司批次ELISA試劑盒供應(yīng),常見ELISA試劑盒享受折扣優(yōu)惠。ELISA試劑盒棋盤試驗(yàn)的操作方法:(1)將抗原按一定的梯度倍比稀釋后,按照ELISA從上到下(或者從左到右)的順序包被。(2)將抗體按一定的梯度倍比稀釋后按照ELISA從左到右或者從上到下加入。(3)二抗的稀釋倍數(shù)是一定的,然后顯色,讀值。
產(chǎn)品名稱:小鼠載脂蛋白H(Apo-H)ELISa免疫檢測試劑盒操作步驟
英文名稱:MouseApolipoproteinH,Apo-HELISA Kit
性狀:盒裝液體。
保存溫度: 2~8℃。
檢測范圍:見說明書靈敏度(以隨貨英文說明書為主)。
說明書:說明書隨貨發(fā)送,您也可以直接我司在線銷售人員索取。
小鼠載脂蛋白H(Apo-H)ELISa免疫檢測試劑盒操作步驟樣品收集、處理及保存方法
1、 血清-----操作過程中避免任何細(xì)胞刺激。使用不含熱原和內(nèi)毒素的試管。收集血液后,1000×g離心10分鐘將血清和紅細(xì)胞迅速小心地分離。
2、 血漿-----EDTA、檸檬酸鹽、肝素血漿可用于檢測。1000×g離心30分鐘去除顆粒。
3、 細(xì)胞上清液---1000×g離心10分鐘去除顆粒和聚合物。
4、 保存------如果樣品不立即使用,應(yīng)將其分成小部分-70 ℃保存,避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
小鼠載脂蛋白H(Apo-H)ELISa免疫檢測試劑盒操作步驟注意事項(xiàng)
1. 試劑應(yīng)按標(biāo)簽說明書儲存,使用前恢復(fù)到室溫。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄,不可保存。
2. 實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中,密封保存,以免變質(zhì)。
3. 不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用。保質(zhì)前使用。
4. 使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時,避免使用帶金屬部分的加樣器。
5. 使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6. 洗滌酶標(biāo)板時應(yīng)充分拍干,不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。
7. 底物A應(yīng)揮發(fā),避免長時間打開蓋子。底物B對光敏感,避免長時間暴露于光下。避免用手接觸,有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。
8. 加入試劑的順序應(yīng)*,以保證所有反應(yīng)板孔溫育的時間一樣。
ELISA Kit for Poly ADP Ribose Polymerase 4 (PARP4)規(guī)格:48T/96T
ELISA Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)規(guī)格:48T/96T
ELISA Kit for Inter Alpha-Globulin Inhibitor H3 (ITIH3)規(guī)格:48T/96T
ELISA Kit for Fibroblast Growth Factor 15 (FGF15)規(guī)格:48T/96T
ELISA Kit for Forkhead Box Protein O3 (FOXO3)規(guī)格:48T/96T
ELISA Kit for Cartilage Associated Protein (CRTAP)規(guī)格:48T/96T
ELISA Kit for Twisted Gastrulation Protein Homolog 1 (TWSG1)規(guī)格:48T/96T
ELISA Kit for omerase Reverse Transcriptase (TERT)規(guī)格:48T/96T
ELISA Kit for BMP Binding Endothelial Regulator (BMPER)規(guī)格:48T/96T
ELISA Kit for Matrix Metalloproteinase 14 (MMP14)規(guī)格:48T/96T
ELISA Kit for BMP Binding Endothelial Regulator (BMPER)規(guī)格:48T/96T
ELISA Kit for Calcyphosine (CAPS)規(guī)格:48T/96T
ELISA Kit for Dysferlin (DYSF)規(guī)格:48T/96T
ELISA Kit for Complement Component 1, Q Subcomponent Like Protein 1 (C1qL1)規(guī)格:48T/96T
ELISA Kit for Complement Component 1, Q Subcomponent C (C1qC)規(guī)格:48T/96T
ELISA Kit for Stanniocalcin 2 (STC2)規(guī)格:48T/96T
ELISA Kit for Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2)規(guī)格:48T/96T
ELISA Kit for Hexosaminidase A Alpha (HEXa)規(guī)格:48T/96T
ELISA Kit for Inhibin Beta B (INHbB)規(guī)格:48T/96T
ELISA Kit for Carboxypeptidase A3, Mast Cell (CPA3)規(guī)格:48T/96T
ELISA Kit for Slit Homolog 1 (Slit1)規(guī)格:48T/96T
ELISA Kit for Survivin (Surv)規(guī)格:48T/96T
ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)規(guī)格:48T/96T
ELISA Kit for ATP Binding Cassette Transporter G2 (ABCG2)規(guī)格:48T/96T
ELISA Kit for Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)規(guī)格:48T/96T
ELISA Kit for Melanoma Inhibitory Activity Protein 1 (MIA1)規(guī)格:48T/96T
ELISA Kit for FK506 Binding Protein Like Protein (FKBPL)規(guī)格:48T/96T
ELISA Kit for Butyrophilin Subfamily 1, Member A1 (BTN1A1)規(guī)格:48T/96T
ELISA Kit for Tenomodulin (TNMD)規(guī)格:48T/96T
ELISA Kit for Carbonic Anhydrase III, Muscle Specific (CA3)規(guī)格:48T/96T
ELISA Kit for Complement Component 1, Q Subcomponent A (C1qA)規(guī)格:48T/96T
ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14)規(guī)格:48T/96T
小鼠載脂蛋白H(Apo-H)ELISa免疫檢測試劑盒操作步驟ELISA Kit for Multimerin 2 (MMRN2)規(guī)格:48T/96T
ELISA Kit for Phosphatidylethanolamine Binding Protein 1 (PEBP1)規(guī)格:48T/96T
ELISA Kit for Hydrogen Voltage Gated Channel Protein 1 (HVCN1)規(guī)格:48T/96T
ELISA Kit for Proline/Serine Rich Coiled Coil protein 1 (PSRC1)規(guī)格:48T/96T
ELISA Kit for ATP Binding Cassette Transporter A3 (ABCA3)規(guī)格:48T/96T
ELISA Kit for Tryptase Beta 2 (TPSb2)規(guī)格:48T/96T
ELISA Kit for Homogentisate-1,2-Dioxygenase (HGD)規(guī)格:48T/96T
ELISA Kit for Carboxypeptidase N1 (CPN1)規(guī)格:48T/96T
ELISA Kit for Proteasome Subunit Beta Type 6 (PSMb6)規(guī)格:48T/96T
ELISA Kit for Translocator Protein (TSPO)規(guī)格:48T/96T
ELISA Kit for Metallothionein 3 (MT3)規(guī)格:48T/96T
ELISA Kit for Diazepam Binding Inhibitor (DBI)規(guī)格:48T/96T
ELISA Kit for Chromogranin B (CHGB)規(guī)格:48T/96T
ELISA Kit for Growth Factor, Augmenter Of Liver Regeneration (GFER)規(guī)格:48T/96T
ELISA Kit for BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI)規(guī)格:48T/96T
ELISA Kit for Transmembrane Protein 2 Like Protein (TMEM2L)規(guī)格:48T/96T
ELISA Kit for Cysteine And Glycine Rich Protein 1 (CSRP1)規(guī)格:48T/96T
ELISA Kit for Dimethylarginine Dimethylaminohydrolase 1 (DDAH1)規(guī)格:48T/96T
ELISA Kit for Coenzyme Q10 Homolog B (COQ10B)規(guī)格:48T/96T
ELISA Kit for Jagged 1 Protein (JAG1)規(guī)格:48T/96T
ELISA Kit for V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB)規(guī)格:48T/96T
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