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小鼠25羥基*3(25(OH)D3/25HVD3)elisa免疫檢測試
小鼠轉(zhuǎn)化生長因子β2(TGFβ2)ELISa免疫檢測試劑盒操作步驟
檢測范圍:
人、綿羊、小鼠、大鼠、豬、兔、山羊、牛、馬、豬、其它動(dòng)物細(xì)胞因子、植物細(xì)胞因子、骨代謝、細(xì)胞凋亡、激素內(nèi)分泌、活性多肽、肝纖維化、自身抗體、血栓與止血、腫瘤、自身抗體科研Elisa檢測試劑盒。
產(chǎn)品名稱:小鼠轉(zhuǎn)化生長因子β2(TGFβ2)ELISa免疫檢測試劑盒操作步驟
英文名稱:Mousetransforminggrowthfactorβ2,TGFβ2ELISA Kit
產(chǎn)品性狀:盒裝液體。
運(yùn)輸條件:2-8℃低溫運(yùn)輸,用干冰或者冰袋低溫運(yùn)輸。
待檢樣本:體液、血清、血漿、細(xì)胞培養(yǎng)上清液、尿液、組織勻漿、心房水標(biāo)本等等。
1、*抗體——高效、靈敏、特異
2、規(guī)范包被操作——吸附均勻,吸附性好,空白值低,孔底透明度高
3、*的優(yōu)化方案——重復(fù)性高,可靠性強(qiáng)
4、技術(shù)服務(wù)要求:專業(yè),規(guī)范,高效
小鼠轉(zhuǎn)化生長因子β2(TGFβ2)ELISa免疫檢測試劑盒操作步驟
原理:ELISA是以免疫學(xué)反應(yīng)為基礎(chǔ),將抗原、抗體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗(yàn)技術(shù)。
ELISA試劑盒檢測類型:
雙抗體夾心法測抗原、雙抗原夾心法測抗體、間接法測抗體、競爭法測抗體、競爭法測抗原、捕獲包被法測抗體、ABS-ELISA法。
類型和操作步驟
ELISA可用于測定抗原,也可用于測定抗體。在這種測定方法中有3種必要的試劑:
①固相的抗原或抗體,
②酶標(biāo)記的抗原或抗體,
③酶作用的底物。
根據(jù)試劑的來源和標(biāo)本的性狀以及檢測的具備條件,可設(shè)計(jì)出各種不同類型的檢測方法。
(一)雙抗體夾心法、
(二)一步法、
(三)間接法測抗體、
(四)競爭法。
技術(shù)原理:
(1). 抗原或抗體的固相化及抗原或抗體的酶標(biāo)記。
(2). 結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性。
(3). 酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性。
(4). 受檢標(biāo)本與固相載體表面的抗原或抗體起反應(yīng)。再加入酶標(biāo)記的抗原或抗體,也通過反應(yīng)而結(jié)合在固相載體上。
(5). 此時(shí)固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例。
(6). 加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析。測定方法具有很高的敏感度(pg-ng/ml水平),并且重復(fù)性好。以免疫學(xué)反應(yīng)為基礎(chǔ),將抗原、抗體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗(yàn)技術(shù)。
ELISA Kit for Angiopoietin 1 (ANGPT1)規(guī)格:48T/96T
ELISA Kit for Angiopoietin 1 (ANGPT1)規(guī)格:48T/96T
ELISA Kit for Gastrin (GT)規(guī)格:48T/96T
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)規(guī)格:48T/96T
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)規(guī)格:48T/96T
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)規(guī)格:48T/96T
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)規(guī)格:48T/96T
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)規(guī)格:48T/96T
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)規(guī)格:48T/96T
ELISA Kit for Connective Tissue Growth Factor (CTGF)規(guī)格:48T/96T
ELISA Kit for Connective Tissue Growth Factor (CTGF)規(guī)格:48T/96T
ELISA Kit for Connective Tissue Growth Factor (CTGF)規(guī)格:48T/96T
ELISA Kit for Connective Tissue Growth Factor (CTGF)規(guī)格:48T/96T
ELISA Kit for Connective Tissue Growth Factor (CTGF)規(guī)格:48T/96T
ELISA Kit for Transferrin Receptor (TFR)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)規(guī)格:48T/96T
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)規(guī)格:48T/96T
ELISA Kit for S100 Calcium Binding Protein (S100)規(guī)格:48T/96T
ELISA Kit for S100 Calcium Binding Protein (S100)規(guī)格:48T/96T
小鼠轉(zhuǎn)化生長因子β2(TGFβ2)ELISa免疫檢測試劑盒操作步驟
ELISA Kit for S100 Calcium Binding Protein (S100)規(guī)格:48T/96T
ELISA Kit for Mucin 1 (MUC1)規(guī)格:48T/96T
ELISA Kit for Mucin 1 (MUC1)規(guī)格:48T/96T
ELISA Kit for Mucin 1 (MUC1)規(guī)格:48T/96T
ELISA Kit for Ciliary Neurotrophic Factor (CNTF)規(guī)格:48T/96T
ELISA Kit for Ciliary Neurotrophic Factor (CNTF)規(guī)格:48T/96T
ELISA Kit for Ciliary Neurotrophic Factor (CNTF)規(guī)格:48T/96T
ELISA Kit for Cadherin, Epithelial (CDHE)規(guī)格:48T/96T
ELISA Kit for Cadherin, Epithelial (CDHE)規(guī)格:48T/96T
ELISA Kit for Cadherin, Epithelial (CDHE)規(guī)格:48T/96T
ELISA Kit for Eosinophil Chemotactic Factor (ECF)規(guī)格:48T/96T
ELISA Kit for Eosinophil Chemotactic Factor (ECF)規(guī)格:48T/96T
ELISA Kit for Eosinophil Chemotactic Factor (ECF)規(guī)格:48T/96T
ELISA Kit for Eosinophil Chemotactic Factor (ECF)規(guī)格:48T/96T
ELISA Kit for Interleukin 8 Receptor Alpha (IL8Ra)規(guī)格:48T/96T
ELISA Kit for Interleukin 8 Receptor Alpha (IL8Ra)規(guī)格:48T/96T
ELISA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)規(guī)格:48T/96T
ELISA Kit for Erythropoietin (EPO)規(guī)格:48T/96T
ELISA Kit for Erythropoietin (EPO)規(guī)格:48T/96T
ELISA Kit for Erythropoietin (EPO)規(guī)格:48T/96T
ELISA Kit for Selectin, Endothelium (SELE)規(guī)格:48T/96T
ELISA Kit for Selectin, Endothelium (SELE)規(guī)格:48T/96T
ELISA Kit for Selectin, Endothelium (SELE)規(guī)格:48T/96T
ELISA Kit for Synuclein Alpha (SNCa)規(guī)格:48T/96T
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