Sigma M7167 M2培養(yǎng)基

sterility:sterile-filtered.

form:liquid.

storage condition:protect from light.

technique(s):cell culture | embryo: suitable.

impurities:endotoxin, tested.

components:phenol red: 0.0106 g/L;sodium pyruvate: 0.0363 g/L;HEPES: 5.42726 g/L;glucose: 1.0 g/L (Dextro);NaHCO3: 0.35 g/L.Application:

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Sigma M7167 M2培養(yǎng)基 M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.