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Anti- NFKB p65抗體現(xiàn)貨供應(yīng)

FROM:ABCAM

Catalog Number : BYK- 0465R       
Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Anti- NFKB p65 antibody現(xiàn)貨供應(yīng)Background: p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. [SUBUNIT] Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. Subcellular location is at Nucleus, Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Tissue Specificity: Spleen; lower level in brain.

Specificity :

• Anti- NFKB p65 is a rabbit monoclonal antibody unconjugated                
• specific for NFKB p65 of mo，hu，rat       
• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  61kDa

Anti- NFKB p65 antibody現(xiàn)貨供應(yīng)Application :  

• Western blotting 1:100-500                
• Immunohistochemistry 1:100-500               
• ELISA 1:500-1000                 
• Optimal working dilutions must be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Anti- NFKB p65 antibody現(xiàn)貨供應(yīng)Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.