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Anti- HPV16 E6 protein抗體產(chǎn)品咨詢

Catalog Number : BYK-0990R      
Quantity size :   0.1ml  (1mg/ml,Lyophilized or Liquid.Buffer=0.01M PBS,ph 7.4 with 10 mg/ml BSA and 0.1% Sodium azide.Reconstitute with 0.1ml sterile distilled water.)

background: Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximay 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Specificity :   

• Anti- HPV16 E6 protein is a rabbit Polyclonal antibody unconjugated                
• specific for HPV16 E6 protein of HPV16
• use for elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  11kDa

Application :                   

• Immunohistocry 1:100-500                
• ELISA= 1:500-1000  
• IHC-P=1:100-500  IHC-F=1:100-500
• IF=1:100-500
• Synthetic peptide from the HPV16-E6 conjugated to KLH
• Optimal working dilutions must be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.