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Anti-GADD153抗體巨額讓利

Catalog Number : BYK-8875R      
Quantity size :   0.1ml  (1mg/ml,Lyophilized or Liquid.Buffer=0.01M PBS,ph 7.4 with 10 mg/ml BSA and 0.1% Sodium azide.Reconstitute with 0.1ml sterile distilled water.)

background: GADD153 (Growth arrest and DNA damage-inducible 153; DNA-damage inducible transcript 3;) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

Specificity :   

• Anti-GADD153 is a rabbit Polyclonal antibody unconjugated                
• specific for GADD153 of Human, Mouse, Rat, Cow,
• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  19kDa

Application :  

• Western blotting=1:100-500                
• Immunohistocry 1:100-500                
• ELISA= 1:500-1000  
• IHC-P=1:100-500  IHC-F=1:100-500

Optimal working dilutions must be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.