產(chǎn)品名稱:10×多聚賴氨酸L型溶液售后服務(wù)
英文名字:10xPoly-L-lysine solution
產(chǎn)品描述
多聚賴氨酸(L型)英文名為Poly-L-lysine,簡稱PLL。本Poly-L-lysine為Poly-L-lysine hydrobromide,分子式為L-Lys-(L-Lys)n-L-Lys•xHBr,分子量為150,000-300,000, Number 25988-63-0。
多聚賴氨酸溶液是廣泛應(yīng)用的組織切片與玻片黏合劑,該多聚陽離子分子與組織切片上的陰離子相互作用會產(chǎn)生較強的黏合力。
本產(chǎn)品為已經(jīng)配制好的多聚賴氨酸L型溶液,并經(jīng)過濾除菌處理,可以直接稀釋后用于細胞或組織培養(yǎng)方面的實驗。多聚賴氨酸溶液,一般的使用濃度為0.01%。本品為10×多聚賴氨酸L型溶液,使用時用滅菌的雙蒸水稀釋至1×后使用。
應(yīng)用
本品適用于組織學(xué),免疫組織化學(xué),凍切片,細胞涂片,原位雜交等實驗中玻片的防脫片處理,以防實驗操作過程中組織掉片。也可用于細胞培養(yǎng),增加細胞貼壁能力。
儲存條件
-20℃保存,避免反復(fù)凍融。
10×多聚賴氨酸L型溶液售后服務(wù)注意事項
1. 每 100ml 已稀釋的多聚賴氨酸溶液可處理 90 張玻片,超過 90 張玻片會影響其黏合力。
2. 包被之前玻片必須保持清潔,必要時用含 1%HCl 的 70%乙醇溶液來清洗。
3. 稀釋過的多聚賴氨酸溶液可放在2-8℃,至少3個月內(nèi)是穩(wěn)定的。
4. 用過的稀釋液再次使用時要過濾,若出現(xiàn)渾濁或染菌應(yīng)丟棄。
5. Poly-L-lysine和Poly-D-lysine都可以用于促進細胞的貼壁生長。Poly-L-lysine可以被某些細胞所消化并吸收,攝入過多的Poly-L-lysine會產(chǎn)生一定的細胞毒性。如果遇到Poly-L-lysine有細胞毒性的情況,可以考慮選購Poly-D-lysine。
6. 為了您的安全和健康,請穿實驗服并戴一次性手套操作。
10×多聚賴氨酸L型溶液售后服務(wù)產(chǎn)品應(yīng)用:
對于一些相對特殊的產(chǎn)品,我們會提供產(chǎn)品說明書,里面會對產(chǎn)品溶解性,及我們所能了解的產(chǎn)品應(yīng)用信息,實驗方案進行描述,您可以作為參考。對于常見試劑,因為應(yīng)用廣泛,一般不提供說明書。如果您在實驗過程中遇到了問題,歡迎與我們討論,我們將盡力幫您解決。
10×多聚賴氨酸L型溶液售后服務(wù)分裝及溶液配制:
您收到貨物后不要自己進行分包,因為分包環(huán)境、包裝材料等因素可能導(dǎo)致分包后的產(chǎn)品變質(zhì);如您有特殊包裝要求,請在訂購時候與我們客服代表闡明,當(dāng)然價格會做適當(dāng)調(diào)整。對于開蓋后,長期未使用的,請務(wù)必重新密封好,建議Parafilm封口膜,并按照相應(yīng)儲存條件使用。如果放置時間過長,超過產(chǎn)品有效期,建議您重新購買,以免影響實驗質(zhì)量。
10×多聚賴氨酸L型溶液售后服務(wù)儲存條件:
大部分的試劑產(chǎn)品是比較穩(wěn)定的,只要您嚴格按照產(chǎn)品包裝或者說明書上的儲存條件進行儲存,*可以保證其在有效期內(nèi)的正常使用。一般常見儲存條件有零下XX攝氏度,2-8攝氏度,常溫等。特別提醒,對于某些酶類制品,如果儲存條件是2-8攝氏度,不要儲存在零下,否則容易失活,對于某些需要嚴格避光,防潮,充氮氣保護的,接觸空氣或者過分暴露非常容易氧化或者受潮變質(zhì)。
低溫儲存產(chǎn)品,運輸途中冷藏問題:
對于需要低溫儲存的產(chǎn)品,我們在運輸時候都會采用冷藏包裝,但是部分由于外界氣溫過高,運輸時間較長,對于普通冷藏包裝,途中可能會溫度升高,這些都是沒有問題的,因為這種普通冷藏只適用于那些短期內(nèi)(2周)高溫依然穩(wěn)定的產(chǎn)品,建議低溫的儲存條件,只是長期儲存時候的條件,您可以放心使用。對于某些特殊的產(chǎn)品,如抗體,我們會采用干冰冷藏包裝,確保全程低溫。公司都對產(chǎn)品性狀進行了嚴格的加速穩(wěn)定測試,行業(yè)經(jīng)驗豐富。
10×多聚賴氨酸L型溶液售后服務(wù)儲存條件:避光、干燥陰涼處封閉貯存,嚴禁與有毒、有害物品混放、混運。本品為非危險 產(chǎn)品可按一般化學(xué)品運輸,輕搬動輕放,防止日曬、雨淋!受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運輸條件比較苛刻。
CFN99589 http://www.chemfaces。。com/natural/Oxypaeoniflorin-CFN99589.html C23H28O12 496.46 39011-91-1 Oxypaeoniflorin Monoterpenoids >=98% 10mg
CFN99591 http://www.chemfaces。。com/natural/Pseudohypericin-CFN99591.html C30H16O9 520.44 55954-61-5 Pseudohypericin Anthraquinones >=98% 20mg
CFN99592 http://www.chemfaces。。com/natural/Rhein-8-O-D-glucopyranoside-CFN99592.html C21H16O11·Ca 484.43 113443-70-2 Rhein-8-glucoside calcium salt Anthraquinones >=98% 20mg
CFN99593 http://www.chemfaces。。com/natural/Sanggenone-C-CFN99593.html C40H36O12 708.71 80651-76-9 Sanggenone C Flavonoids >=98% 20mg
CFN99595 http://www.chemfaces。。com/natural/Senkyunolide-H-CFN99595.html C12H16O4 224.3 94596-27-7 Senkyunolide H Miscellaneous >=98% 20mg
CFN99597 http://www.chemfaces。。com/natural/Sennidin-A-CFN99597.html C30H18O10 538.46 641-12-3 Sennidin A Anthraquinones >=98% 20mg
CFN99598 http://www.chemfaces。。com/natural/Sennidin-B-CFN99598.html C30H18O10 538.46 517-44-2 Sennidin B Anthraquinones >=98% 20mg
CFN99599 http://www.chemfaces。。com/natural/Sinensetin-CFN99599.html C20H20O7 372.37 2306-27-6 Sinensetin Flavonoids >=98% 20mg
CFN99703 http://www.chemfaces。。com/natural/Cyperone-CFN99703.html C15H22O 218.33 473-08-5 alpha-Cyperone Sesquiterpenoids >=98% 20mg
required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP. Phosphorylated by NUAK1 at Ser-15 and Ser-392; was initially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1 (PubMed:21317932). It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.
Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.
May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.
Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. 10×多聚賴氨酸L型溶液售后服務(wù)Ubiquitinated by TRIM24, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner.
Monomethylated at Lys-372 by SETD7, leading to