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Nano LC System

Nano LC System 產(chǎn)品詳情

Nano LC System

Mieux^TM

Introducing the NewMieux!

Cutting edge pumping technology for outstanding stability andreproducibility

The Mieux System is an innovative system developed for nano-LCfor MS. This system is best suited for proteome, metabolome, anddrug metabolism analyses, as well as for high-separation andhigh-sensitivity analysis.

Gradient pumping exceeding50 nL/min.
Our unique pumping system can achieve a gradient of 50 nL/min.Direct pumping without using the splitter ensures less change inthe flow rate under conditions which produce pressure fluctuations(associated with column exchange), offering stable reproducibilityａnd makes it unnecessary to adjust the split ratio.

Features

Unique pumping system, enabling a gradient of 50 nL/min withoutusing the splitter
Pressure monitor enables full-time monitoring of pumping state
Various programs (auto flash, auto refill, auto drain) enablethe System’s high-speed equilibrium and full-automatic pumping
Proprietary software enables full-automatic measurement for 1Dto 2D systems
Combining this system with the Spotter Mieux SP (OFF Line/LCMS)for MALDI-TOF MS enables spotting to a target plate andmicro-fractionation
Connectable to other types of MS
Unique nano-spray stage and nano-spray column holder available(optional)

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Connectable to other mass spectrometers

Applied Biosystems / MDS CIEX · Micromass· BRUKER DALTONICS · Thermo Fisher, and others

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Analysis data sample (Mieux+Q-Star Elite, t-BSA 50fmol Coverage 70%)

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Nano-spray stages and nano-spray holders for various MS systems

Nano Spray Stage for LCQ	Nano Spray Stage for LTQ	Nano Spray Stage Holder for LTQ, Esquire, Accu TOF
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Mieux System

		Configuration
Mieux-I	Manual injection system. This system enablesdesalting condensation manually using the trap column.	Gradient pump, nano-injection valve withactuator, control software, PC, analytical column, trap column
Mieux-II	Auto injection system, which enables gradientanalysis after automatic sample injection and desalting.	Gradient pump, single pump, auto sampler,trap column valve, control software, PC, analytical column, trapcolumn (*)
Mieux-III	Direct injection system, which has beendeveloped for high-sensitivity detection of phosphoric peptides.	Gradient pump, single pump, auto sampler(micro specifications), trap column valve, control software, PC,phosphoric peptide analytical column, trap column (*)
Mieux-IV	Two-dimensional auto injection system. Thison-line two-dimensional system performs the single dimension ionexchange in a stepwise manner and two-dimensional reverse-phaseseparation and elution based on the gradient set-up.	Gradient pump, single pump, auto sampler,trap column valve, ion exchange column valve, control software, PC,ion exchange column, analytical column, and trap column (*)
Mieux-V	Two-dimensional auto injection system. Thison-line two-dimensional system performs analysis based on thegradient set-up in one and two dimensions.	Gradient pumps ×2, single pumps ×2, autosampler, trap column valve, ion exchange column valve ×2, controlsoftware, PC, ion exchange column, analytical column, trap column(*)

* Single pump is installed in "Mieux"

? Optional functions available for Phosphoric Peptide Analysisin each systems of Mieux-I, II, IV, and V.

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User-friendly dedicated software

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Pumping flow schematic

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Various Columns

Various column packing, Nano-LC Trap column,Nano-LC capillary column, Micro, semi-micro, conventional,preperative isolation column

Nano-LC capillary column

Size number	Column size
2-*	0.2mm I.D × *mmL
1.5-*	0.15mm I.D × *mmL
1-*	0.1mm I.D × *mmL
1-* 0.75-*	0.075mm I.D × *mmL

Nano-LC trap column

Size number	Column size
0.5-1	0.5mm I.D × 1mmL
0.8-3	0.8mm I.D × 3mmL

* Same price regardless of size Please ask the typeof column packing and size which you require.

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Mieux Series

Mieux

Gradient Nano Pump

Remarks
Auto injection system : single pump included
Manual injection system : single pump not included (gradient pumponly)

Mieux AS

Auto Sampler

Mieux AP

Single Nano Pump

Mieux SP

MALDI Spotter
Nano Micro-Fraction Collector

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Mieux Spotter System For MALDI-TOFMS

Example:
1504 spottings on target plate of Applied Bio 4800

By connecting with Mieux,this system enables stable spotting at nL level.

Continuous dispensing to multiple plates

Mixed operation of various mfr’s MALDI plates and Microplatesis possible

Simultaneous automatic addition of matrix solution

Sample eluate and matrix added simultaneously from the needletip by using the dedicated nano pump (Mieux AP)
Plate is not damaged by spotting because a non-contact needleis used
Spotting of sample solution is possible at 100 nL/min by usinga dedicated needle

Operation control by dedicated software

Integrated control of the SP system together with the MieuxNano LC system
Dispensing time can be set freely, starting from 5 seconds
Dispensing is possible using an external signal from thedetector
Automatic dispensing time calculation function based on theentered analytical time and number of dispensing steps

Configuration	Mieux SP-I	Mieux-I system, Mieux SP, Mieux AP
	Mieux SP-II	Mieux-II system, Mieux SP, Mieux AP
	Mieux SP-III	Mieux-II system, Mieux SP, Mieux AP Mieux-IIIsystem, Mieux SP, Mieux AP
	Mieux SP-IV	Mieux-IV system, Mieux SP, Mieux AP
	Mieux SP-V	Mieux-V system, Mieux SP, MieuxAP

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Specifications

Nano gradient pump(Mieux)

Pumping type	One high-pressure double syringe pumpingsystem installed One high-pressure single syringe pumping system installed
Syringe volume	220uL
Operation control	with PC operating dedicated software
Communication	RS-232C
Flow rate range	High-pressure double syringe pumping type 1 nL/min to 200,000 nL/min in 1 nL step (Gradient 50 nL/min to 1000nL/min) High-pressure single syringe pumping type 1 nL/min to 200,000 nL/min in 1 nL step
Working pressure	0.0 to 20.0MPa
Pressure indication	0.0 to 50.0MPa in the 0.1 MPa step
Functions	Auto flush, auto refill, auto drain
Backside terminal	RS-232C, external program start terminal,pressure output (2-ch)
Power supply	AC100V to 240V
Size	W300 × H210 × D500mm
Power consumption	Max 200 VA

Nano pump (MieuxAP)

Pumping type	High-pressure syringe pumping system
Syringe volume	220uL
Operation control	with PC operating dedicated software
Communication	RS-232C
Flow range	1 nL/min to 100,000 nL/min in 1 nL step
Working pressure	0.0 to 15.0Mpa
Pressure indication	0.0 to 50.0MPa in 0.1 MPa step
Functions	Auto flush, auto refill, auto drain
Backside terminal	RS-232C, external program start terminal,pressure output
Power supply	AC100V to 240V
Size	W140 × H160 × D320mm
Power consumption	Max 150 VA

Auto injector (Mieux AS) (standardspecifications)

Sample injection method	Full loop, partial loop, micro pickup
Sample injection rate	0.1 to 100uL
Sample rack	48 vial containers, 96 plates (Low,High), 384(Low)
Injection accuracy	Full loop RSD≦0.3 Partial loop RSD≦0.5%(＞5μL) Micro pickup RSD≦1.0%(＞5μL) (*For the micro specifications, contact us separately. )
Operation control	With PC operating dedicated software or fromthe keypad on the main body
Communication	RS-232C
Cooling function	Min 4 deg.C (room temperature -16 deg. C)
Power supply	AC115V(50/60Hz), AC230V(50/60Hz)
Size	W280 × H400 × D440mm
Power consumption	Max. 250VA

*Descriptions in the catalog are subject tochange without notice.

Nano HPLC fraction collector (MieuxSP)

Applicable plate	MALDI Plate	Applied Biosystems/MDS SCIEX Max 8 plates Bruker Daltonics Max 4 plates Waters/Micromass Max 4 plates
	Microplate	96 well plate(Low, High), 384 well plate(Low)
Spotting method		Contact or non-contact spotting, Matrix mixed spotting without needle contact (Note: Matrix fluid feed pump is optional.)
Probe		Two-layer (PTFE and SUS) structure (Sample is center and the matrix solution eluting from the outside)
Allowable dispensing flow range		100nL/min. ～
Matrix flow rate range		100nL/min. ～
Spotting mode		Continuous mode (continuous spotting every fixed giveninterval) Continuous spotting by external pulse signal
Needle Cleaning		Automatic needle cleaning by washingsolvent Waste solution port provided
Spotting direction		Straight, zigzagging
Operation control		PC operating dedicated software
Communication		RS-232C
Power supply		AC100 to AC240
Size		W500 × H330 × D490mm
Weight		Approx. 39 kg

(Note: Matrix fluid feedpump is optional.)

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