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1M HEPES 溶液(細(xì)胞培養(yǎng)級(jí))操作步驟儲(chǔ)存條件：避光、干燥陰涼處封閉貯存,嚴(yán)禁與有毒、有害物品混放、混運(yùn)。本品為非危險(xiǎn) 產(chǎn)品可按一般化學(xué)品運(yùn)輸,輕搬動(dòng)輕放,防止日曬、雨淋！受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運(yùn)輸條件比較苛刻。
產(chǎn)品名稱：1M HEPES 溶液(細(xì)胞培養(yǎng)級(jí))操作步驟
英文名字：1M Hepes Solution

產(chǎn)品描述

HEPES即 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid，中文名稱N-(2-羥乙基)- N'-2磺酸，分子式為C8H18N2O4S，分子量為238.31。

1M HEPES 溶液(細(xì)胞培養(yǎng)級(jí))操作步驟產(chǎn)品優(yōu)勢(shì)

無(wú)細(xì)胞毒性作用。
一種非離子兩性緩沖劑，能有效控制pH在6.8～8.2范圍，尤其在pH7.2～7.4具有較好的緩沖能力。
開(kāi)放式培養(yǎng)或細(xì)胞觀察時(shí)能維持較恒定PH值。
已經(jīng)過(guò)無(wú)菌處理，直接加入到培養(yǎng)基中即可

儲(chǔ)存條件

2-8℃。

注意事項(xiàng)

為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

1M HEPES 溶液(細(xì)胞培養(yǎng)級(jí))操作步驟儲(chǔ)存條件：
大部分的試劑產(chǎn)品是比較穩(wěn)定的，只要您嚴(yán)格按照產(chǎn)品包裝或者說(shuō)明書(shū)上的儲(chǔ)存條件進(jìn)行儲(chǔ)存，*可以保證其在有效期內(nèi)的正常使用。一般常見(jiàn)儲(chǔ)存條件有零下XX攝氏度，2-8攝氏度，常溫等。特別提醒，對(duì)于某些酶類制品，如果儲(chǔ)存條件是2-8攝氏度，不要儲(chǔ)存在零下，否則容易失活，對(duì)于某些需要嚴(yán)格避光，防潮，充氮?dú)獗Ｗo(hù)的，接觸空氣或者過(guò)分暴露非常容易氧化或者受潮變質(zhì)。
低溫儲(chǔ)存產(chǎn)品，運(yùn)輸途中冷藏問(wèn)題：
對(duì)于需要低溫儲(chǔ)存的產(chǎn)品，我們?cè)谶\(yùn)輸時(shí)候都會(huì)采用冷藏包裝，但是部分由于外界氣溫過(guò)高，運(yùn)輸時(shí)間較長(zhǎng)，對(duì)于普通冷藏包裝，途中可能會(huì)溫度升高，這些都是沒(méi)有問(wèn)題的，因?yàn)檫@種普通冷藏只適用于那些短期內(nèi)（2周）高溫依然穩(wěn)定的產(chǎn)品，建議低溫的儲(chǔ)存條件，只是長(zhǎng)期儲(chǔ)存時(shí)候的條件，您可以放心使用。對(duì)于某些特殊的產(chǎn)品，如抗體，我們會(huì)采用干冰冷藏包裝，確保全程低溫。公司都對(duì)產(chǎn)品性狀進(jìn)行了嚴(yán)格的加速穩(wěn)定測(cè)試，行業(yè)經(jīng)驗(yàn)豐富。
1M HEPES 溶液(細(xì)胞培養(yǎng)級(jí))操作步驟產(chǎn)品應(yīng)用：
對(duì)于一些相對(duì)特殊的產(chǎn)品，我們會(huì)提供產(chǎn)品說(shuō)明書(shū)，里面會(huì)對(duì)產(chǎn)品溶解性，及我們所能了解的產(chǎn)品應(yīng)用信息，實(shí)驗(yàn)方案進(jìn)行描述，您可以作為參考。對(duì)于常見(jiàn)試劑，因?yàn)閼?yīng)用廣泛，一般不提供說(shuō)明書(shū)。如果您在實(shí)驗(yàn)過(guò)程中遇到了問(wèn)題，歡迎與我們討論，我們將盡力幫您解決。
1M HEPES 溶液(細(xì)胞培養(yǎng)級(jí))操作步驟分裝及溶液配制：
您收到貨物后不要自己進(jìn)行分包，因?yàn)榉职h(huán)境、包裝材料等因素可能導(dǎo)致分包后的產(chǎn)品變質(zhì)；如您有特殊包裝要求，請(qǐng)?jiān)谟嗁?gòu)時(shí)候與我們客服代表闡明，當(dāng)然價(jià)格會(huì)做適當(dāng)調(diào)整。對(duì)于開(kāi)蓋后，長(zhǎng)期未使用的，請(qǐng)務(wù)必重新密封好，建議Parafilm封口膜，并按照相應(yīng)儲(chǔ)存條件使用。如果放置時(shí)間過(guò)長(zhǎng)，超過(guò)產(chǎn)品有效期，建議您重新購(gòu)買，以免影響實(shí)驗(yàn)質(zhì)量。
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CFN99564   http://www.chemfaces。。ｃｏｍ/natural/Coptisine-chloride-CFN99564.html   C19H14NO4Cl   355.77   6020-18-4   Coptisine chloride   Alkaloids   >=98%   20mg
CFN99566   http://www.chemfaces。。ｃｏｍ/natural/Picroside-II-CFN99566.html   C23H28O13   512.47   39012-20-9   Picroside II   Iridoids   >=98%   20mg
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CFN99612   http://www.chemfaces。。ｃｏｍ/natural/Proline-CFN99612.html   C5H9NO2   115.1    147-85-3   Proline   Alkaloids   >=99%   200mg
CFN99573   http://www.chemfaces。。ｃｏｍ/natural/Filixic-acid-ABA-CFN99573.html   C32H36O12   612.63   38226-84-5   Filixic acid ABA   Phenols   >=98%   10mg
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CFN99575   http://www.chemfaces。。ｃｏｍ/natural/Licochalcone-A-CFN99575.html   C21H22O4   338.40   58749-22-7   Licochalcone A   Chalcones   >=98%   20mg
CFN99576   http://www.chemfaces。。ｃｏｍ/natural/Licochalcone-B-CFN99576.html   C16H14O5   286.28   58749-23-8   Licochalcone B   Chalcones   >=98%   20mg

Interacts with CDK5 in neurons. Interacts with AURKB, UHRF2 and NOC2L. Interacts (via N-terminus) with PTK2/FAK1; this promotes ubiquitination by MDM2. Interacts with PTK2B/PYK2; this promotes ubiquitination by MDM2. Interacts with PRKCG. Interacts with human cytomegalovirus/HHV-5 protein UL123.
Subcellular Location : Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Note=Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2.
Isoform 1: Nucleus. Cytoplasm. Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.
Isoform 2: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.
Isoform 3: Nucleus. Cytoplasm. Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells.
Isoform 4: Nucleus. Cytoplasm. Note=Predominantly nuclear but translocates to the cytoplasm following cell stress.
Isoform 7: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.
Isoform 8: Nucleus. Cytoplasm. Note=Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli.
Isoform 9: Cytoplasm.
1M HEPES 溶液(細(xì)胞培養(yǎng)級(jí))操作步驟Tissue Specificity : Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues