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DAPI溶液(1mg/ml)操作步驟*,*,庫存現(xiàn)貨,無效果,免費退款。公司現(xiàn)貨銷售,量大折扣,咨詢。詳細(xì)信息如下。
產(chǎn)品名稱 | 英文名稱 | 包裝 |
DAPI溶液(1mg/ml)操作步驟 | DAPI solution (1MG/ML) | 可根據(jù)客戶要求訂制 |
DAPI溶液(1mg/ml)操作步驟產(chǎn)品描述
DAPI溶液(1mg/ml)即 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI),中文名為4’,6-二脒基-2-苯基吲哚。本產(chǎn)品是一種可以穿透細(xì)胞膜的DNA鏈藍色熒光染料,DAPI染色常用于細(xì)胞凋亡檢測,染色后用熒光顯微鏡觀察或流式細(xì)胞儀檢測。DAPI也常用于普通的細(xì)胞核染色以及某些特定情況下的雙鏈DNA染色。
產(chǎn)品優(yōu)勢
本產(chǎn)品為DAPI水溶液,純度≥90%,濃度為1mg/ml。使用時根據(jù)實驗不同直接將本產(chǎn)品用相應(yīng)溶液稀釋到工作濃度。
儲存條件
−20℃避光保存,有效期至少2年。
DAPI溶液(1mg/ml)操作步驟注意事項?
DAPI對人體有一定刺激性,請注意適當(dāng)防護。
熒光染料都存在淬滅的問題,建議染色后盡量當(dāng)天完成檢測。
為減緩熒光淬滅可以使用抗熒光淬滅封片液。
為了您的安全和健康,請穿實驗服并戴一次性手套操作。
DAPI溶液(1mg/ml)操作步驟分子生物學(xué)領(lǐng)域:
涉及核酸純化、常用分子生物學(xué)試劑、PCR等相關(guān)試劑盒。
分子生物學(xué)試劑:擁有的價格優(yōu)勢,品質(zhì)可信賴,市場占比龐大。
DAPI溶液(1mg/ml)操作步驟收貨注意事項:
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optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Proliferation Marker
Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA DAPI溶液(1mg/ml)操作步驟synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.
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